{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ganez-Zapater A"],"funding":["cancerfonden","European Research Council","carl tryggers stiftelse för vetenskaplig forskning","vetenskapsrådet","stockholms universitet","Stockholm University"],"pagination":["463-484"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8960663"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["297(2)"],"pubmed_abstract":["BRG1 and BRM are ATPase core subunits of the human SWI/SNF chromatin remodelling complexes mainly associated with transcriptional initiation. They also have a role in alternative splicing, which has been shown for BRM-containing SWI/SNF complexes at a few genes. Here, we have identified a subset of genes which harbour alternative exons that are affected by SWI/SNF ATPases by expressing the ATPases BRG1 and BRM in C33A cells, a BRG1- and BRM-deficient cell line, and analysed the effect on splicing by RNA sequencing. BRG1- and BRM-affected sub-sets of genes favouring both exon inclusion and exon skipping, with only a minor overlap between the ATPase. Some of the changes in alternative splicing induced by BRG1 and BRM expression did not require the ATPase activity. The BRG1-ATPase independent"],"journal":["Molecular genetics and genomics : MGG"],"pubmed_title":["The SWI/SNF subunit BRG1 affects alternative splicing by changing RNA binding factor interactions with nascent RNA."],"pmcid":["PMC8960663"],"funding_grant_id":["CAN 2016/460","2015–04553","2015-04553","CTS15:568","758397"],"pubmed_authors":["Visa N","Guo Y","Ganez-Zapater A","Mackowiak SD","Tarbier M","Ostlund Farrants AK","Jordan-Pla A","Friedlander MR"],"additional_accession":[]},"is_claimable":false,"name":"The SWI/SNF subunit BRG1 affects alternative splicing by changing RNA binding factor interactions with nascent RNA.","description":"BRG1 and BRM are ATPase core subunits of the human SWI/SNF chromatin remodelling complexes mainly associated with transcriptional initiation. They also have a role in alternative splicing, which has been shown for BRM-containing SWI/SNF complexes at a few genes. Here, we have identified a subset of genes which harbour alternative exons that are affected by SWI/SNF ATPases by expressing the ATPases BRG1 and BRM in C33A cells, a BRG1- and BRM-deficient cell line, and analysed the effect on splicing by RNA sequencing. BRG1- and BRM-affected sub-sets of genes favouring both exon inclusion and exon skipping, with only a minor overlap between the ATPase. Some of the changes in alternative splicing induced by BRG1 and BRM expression did not require the ATPase activity. The BRG1-ATPase independent","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-05-31T21:03:29.623Z","creation":"2025-04-04T21:43:41.862Z"},"accession":"S-EPMC8960663","cross_references":{"pubmed":["35187582"],"doi":["10.1007/s00438-022-01863-9"]}}