biostudies-literatureGanez-Zapater AcancerfondenEuropean Research Councilcarl tryggers stiftelse för vetenskaplig forskningvetenskapsrådetstockholms universitetStockholm University463-484https://www.ebi.ac.uk/biostudies/studies/S-EPMC8960663biostudies-literatureUnknown297(2)BRG1 and BRM are ATPase core subunits of the human SWI/SNF chromatin remodelling complexes mainly associated with transcriptional initiation. They also have a role in alternative splicing, which has been shown for BRM-containing SWI/SNF complexes at a few genes. Here, we have identified a subset of genes which harbour alternative exons that are affected by SWI/SNF ATPases by expressing the ATPases BRG1 and BRM in C33A cells, a BRG1- and BRM-deficient cell line, and analysed the effect on splicing by RNA sequencing. BRG1- and BRM-affected sub-sets of genes favouring both exon inclusion and exon skipping, with only a minor overlap between the ATPase. Some of the changes in alternative splicing induced by BRG1 and BRM expression did not require the ATPase activity. The BRG1-ATPase independentMolecular genetics and genomics : MGGThe SWI/SNF subunit BRG1 affects alternative splicing by changing RNA binding factor interactions with nascent RNA.PMC8960663CAN 2016/4602015–045532015-04553CTS15:568758397Visa NGuo YGanez-Zapater AMackowiak SDTarbier MOstlund Farrants AKJordan-Pla AFriedlander MRfalseThe SWI/SNF subunit BRG1 affects alternative splicing by changing RNA binding factor interactions with nascent RNA.BRG1 and BRM are ATPase core subunits of the human SWI/SNF chromatin remodelling complexes mainly associated with transcriptional initiation. They also have a role in alternative splicing, which has been shown for BRM-containing SWI/SNF complexes at a few genes. Here, we have identified a subset of genes which harbour alternative exons that are affected by SWI/SNF ATPases by expressing the ATPases BRG1 and BRM in C33A cells, a BRG1- and BRM-deficient cell line, and analysed the effect on splicing by RNA sequencing. BRG1- and BRM-affected sub-sets of genes favouring both exon inclusion and exon skipping, with only a minor overlap between the ATPase. Some of the changes in alternative splicing induced by BRG1 and BRM expression did not require the ATPase activity. The BRG1-ATPase independent2022-01-01T00:00:00Z2022 Mar2026-05-31T21:03:29.623Z2025-04-04T21:43:41.862ZS-EPMC89606633518758210.1007/s00438-022-01863-9