{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(1)"],"submitter":["Song G"],"pubmed_abstract":["Intrahepatic cholangiocarcinoma (iCCA) is a highly heterogeneous cancer with limited understanding of its classification and tumor microenvironment. Here, by performing single-cell RNA sequencing on 144,878 cells from 14 pairs of iCCA tumors and non-tumor liver tissues, we find that S100P and SPP1 are two markers for iCCA perihilar large duct type (iCCA<sup>phl</sup>) and peripheral small duct type (iCCA<sup>pps</sup>). S100P + SPP1- iCCA<sup>phl</sup> has significantly reduced levels of infiltrating CD4<sup>+</sup> T cells, CD56<sup>+</sup> NK cells, and increased CCL18<sup>+</sup> macrophages and PD1<sup>+</sup>CD8<sup>+</sup> T cells compared to S100P-SPP1 + iCCA<sup>pps</sup>. The transcription factor CREB3L1 is identified to regulate the S100P expression and promote tumor cell invasion. S100P-SPP1 + iCCA<sup>pps</sup> has significantly more SPP1<sup>+</sup> macrophage infiltration, less aggressiveness and better survival than S100P + SPP1- iCCA<sup>phl</sup>. Moreover, S100P-SPP1 + iCCA<sup>pps</sup> harbors tumor cells at different status of differentiation, such as ALB + hepatocyte differentiation and ID3+ stemness. Our study extends the understanding of the diversity of tumor cells in iCCA."],"journal":["Nature communications"],"pagination":["1642"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8960779"],"repository":["biostudies-literature"],"pubmed_title":["Single-cell transcriptomic analysis suggests two molecularly subtypes of intrahepatic cholangiocarcinoma."],"pmcid":["PMC8960779"],"pubmed_authors":["Zhang J","Lin Y","Jiang S","Zhang S","Cheng Y","Shi Y","Liu Y","Rao D","Fan J","Wang X","Wu Y","Zhou J","Li J","Xi R","Song G","Gao Q","Ji S","Huang S","Cao Y","Zhang X","Ke A","Meng L","Ma J","Lin J","Ji Y","Yang S"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell transcriptomic analysis suggests two molecularly subtypes of intrahepatic cholangiocarcinoma.","description":"Intrahepatic cholangiocarcinoma (iCCA) is a highly heterogeneous cancer with limited understanding of its classification and tumor microenvironment. Here, by performing single-cell RNA sequencing on 144,878 cells from 14 pairs of iCCA tumors and non-tumor liver tissues, we find that S100P and SPP1 are two markers for iCCA perihilar large duct type (iCCA<sup>phl</sup>) and peripheral small duct type (iCCA<sup>pps</sup>). S100P + SPP1- iCCA<sup>phl</sup> has significantly reduced levels of infiltrating CD4<sup>+</sup> T cells, CD56<sup>+</sup> NK cells, and increased CCL18<sup>+</sup> macrophages and PD1<sup>+</sup>CD8<sup>+</sup> T cells compared to S100P-SPP1 + iCCA<sup>pps</sup>. The transcription factor CREB3L1 is identified to regulate the S100P expression and promote tumor cell invasion. S100P-SPP1 + iCCA<sup>pps</sup> has significantly more SPP1<sup>+</sup> macrophage infiltration, less aggressiveness and better survival than S100P + SPP1- iCCA<sup>phl</sup>. Moreover, S100P-SPP1 + iCCA<sup>pps</sup> harbors tumor cells at different status of differentiation, such as ALB + hepatocyte differentiation and ID3+ stemness. Our study extends the understanding of the diversity of tumor cells in iCCA.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-18T23:46:57.685Z","creation":"2025-02-19T04:57:25.276Z"},"accession":"S-EPMC8960779","cross_references":{"pubmed":["35347134"],"doi":["10.1038/s41467-022-29164-0"]}}