{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Furtwangler B"],"funding":["Novo Nordisk Fonden"],"pagination":["100219"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8961214"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(4)"],"pubmed_abstract":["In the young field of single-cell proteomics (scMS), there is a great need for improved global proteome characterization, both in terms of proteins quantified per cell and quantitative performance thereof. The recently introduced real-time search (RTS) on the Orbitrap Eclipse Tribrid mass spectrometer in combination with SPS-MS3 acquisition has been shown to be beneficial for the measurement of samples that are multiplexed using isobaric tags. Multiplexed scMS requires high ion injection times and high-resolution spectra to quantify the single-cell signal; however, the carrier channel facilitates peptide identification and thus offers the opportunity for fast on-the-fly precursor filtering before committing to the time-intensive quantification scan. Here, we compared classical MS2 acquisit"],"journal":["Molecular & cellular proteomics : MCP"],"pubmed_title":["Real-Time Search-Assisted Acquisition on a Tribrid Mass Spectrometer Improves Coverage in Multiplexed Single-Cell Proteomics."],"pmcid":["PMC8961214"],"funding_grant_id":["NNF21SA0073733"],"pubmed_authors":["Porse BT","Furtwangler B","Zabrouskov V","Lopez-Ferrer D","Uresin N","Motamedchaboki K","Schoof EM","Huguet R"],"additional_accession":[]},"is_claimable":false,"name":"Real-Time Search-Assisted Acquisition on a Tribrid Mass Spectrometer Improves Coverage in Multiplexed Single-Cell Proteomics.","description":"In the young field of single-cell proteomics (scMS), there is a great need for improved global proteome characterization, both in terms of proteins quantified per cell and quantitative performance thereof. The recently introduced real-time search (RTS) on the Orbitrap Eclipse Tribrid mass spectrometer in combination with SPS-MS3 acquisition has been shown to be beneficial for the measurement of samples that are multiplexed using isobaric tags. Multiplexed scMS requires high ion injection times and high-resolution spectra to quantify the single-cell signal; however, the carrier channel facilitates peptide identification and thus offers the opportunity for fast on-the-fly precursor filtering before committing to the time-intensive quantification scan. Here, we compared classical MS2 acquisit","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2026-05-30T20:57:33.451Z","creation":"2025-04-04T10:02:15.732Z"},"accession":"S-EPMC8961214","cross_references":{"pubmed":["35219906"],"doi":["10.1016/j.mcpro.2022.100219"]}}