biostudies-literatureUnknown18Dong HA personalized medication regimen provides precise treatment for an individual and can be guided by pre-clinical drug screening. The economical and high-efficiency simulation of the liver tumor microenvironment (TME) in a drug-screening model has high value yet challenging to accomplish. Herein, we propose a simulation of the liver TME with suspended alginate-gelatin hydrogel capsules encapsulating patient-derived liver tumor multicellular clusters, and the culture of patient-derived tumor organoids(PDTOs) for personalized pre-clinical drug screening. The hydrogel capsule offers a 3D matrix environment with mechanical and biological properties similar to those of the liver <i>in vivo</i>. As a result, 18 of the 28 patient-derived multicellular clusters were successfully cultured as PDTOs. Bioactive materials164-177https://www.ebi.ac.uk/biostudies/studies/S-EPMC8961426biostudies-literatureCulture of patient-derived multicellular clusters in suspended hydrogel capsules for pre-clinical personalized drug screening.PMC8961426Yang XBian SLi ZSong PDong HXie HLi TZheng SZhang MSong GSong WfalseCulture of patient-derived multicellular clusters in suspended hydrogel capsules for pre-clinical personalized drug screening.A personalized medication regimen provides precise treatment for an individual and can be guided by pre-clinical drug screening. The economical and high-efficiency simulation of the liver tumor microenvironment (TME) in a drug-screening model has high value yet challenging to accomplish. Herein, we propose a simulation of the liver TME with suspended alginate-gelatin hydrogel capsules encapsulating patient-derived liver tumor multicellular clusters, and the culture of patient-derived tumor organoids(PDTOs) for personalized pre-clinical drug screening. The hydrogel capsule offers a 3D matrix environment with mechanical and biological properties similar to those of the liver <i>in vivo</i>. As a result, 18 of the 28 patient-derived multicellular clusters were successfully cultured as PDTOs. 2022-01-01T00:00:00Z2022 Dec2025-04-04T23:16:36.877Z2025-04-04T23:16:36.877ZS-EPMC89614263538716810.1016/j.bioactmat.2022.03.020