{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ucar B"],"funding":["Austrian Science Fund FWF"],"pagination":["163"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8961638"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(2)"],"pubmed_abstract":["The accumulation of α-synuclein (α-syn) in the brain plays a role in synucleinopathies and it is hypothesized to spread in a prion-like fashion between connected brain regions. In the present study, we aim to investigate this spreading in well-characterized sagittal organotypic whole brain slices taken from postnatal wild type (WT) and transgenic mice overexpressing human α-syn under the promoter of proteolipid protein (PLP). Collagen hydrogels were loaded with monomers of human α-syn, as well as human and mouse pre-formed fibrils (PFFs), to allow local application and slow release. The spreading of α-syn was evaluated in different brain regions by immunohistochemistry for total α-syn and α-syn phosphorylated at the serine129 position (α-syn-P). The application of human and mouse PFFs of α"],"journal":["Biomolecules"],"pubmed_title":["Spreading of Aggregated α-Synuclein in Sagittal Organotypic Mouse Brain Slices."],"pmcid":["PMC8961638"],"funding_grant_id":["P32558-B"],"pubmed_authors":["Stefanova N","Ucar B","Humpel C"],"additional_accession":[]},"is_claimable":false,"name":"Spreading of Aggregated α-Synuclein in Sagittal Organotypic Mouse Brain Slices.","description":"The accumulation of α-synuclein (α-syn) in the brain plays a role in synucleinopathies and it is hypothesized to spread in a prion-like fashion between connected brain regions. In the present study, we aim to investigate this spreading in well-characterized sagittal organotypic whole brain slices taken from postnatal wild type (WT) and transgenic mice overexpressing human α-syn under the promoter of proteolipid protein (PLP). Collagen hydrogels were loaded with monomers of human α-syn, as well as human and mouse pre-formed fibrils (PFFs), to allow local application and slow release. The spreading of α-syn was evaluated in different brain regions by immunohistochemistry for total α-syn and α-syn phosphorylated at the serine129 position (α-syn-P). The application of human and mouse PFFs of α","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2025-04-19T13:00:32.979Z","creation":"2025-04-19T13:00:32.979Z"},"accession":"S-EPMC8961638","cross_references":{"pubmed":["35204664"],"doi":["10.3390/biom12020163"]}}