{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Babenko VN"],"funding":["Russian Science Foundation"],"pagination":["277"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8961644"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(2)"],"pubmed_abstract":["We performed transcriptome analysis in the hippocampus 24 h after lipopolysaccharide (LPS) administration. We observed glial-specific genes, comprised of two-thirds of all differentially expressed genes (DEGs). We found microglial DEGs that were the most numerous in LPS group. On the contrary, differential alternative splicing (DAS) analysis revealed the most numerous DAS events in astrocytes. Besides, we observed distinct major isoform switching in the <i>Ptbp1</i> gene, with skipping of exon 8 in LPS group. <i>Ptbp1</i> usually considered a pluripotency sustaining agent in brain embryonic development, according to the previous studies. Analyzing the splicing tune-up upon LPS exposure, we came to a supposition that the short <i>Ptbp1</i> isoform de-represses immune-specific response by <i>Ptbp1</i> adjusted splicing architecture. Additionally, the <i>Ptbp3</i> (<i>NOD1</i>) immune-specific splicing factor has apparently been de-repressed by the <i>Ptbp1</i> short isoform in glial cells. Notably, both the <i>Ptbp1</i> and <i>Ptbp3</i> genes express primarily in microglial/endothelial brain cells. We also report immune-related genes, altering their major isoforms upon LPS exposure. The results revealed immune modulating role of alternative splicing in brain."],"journal":["Biomolecules"],"pubmed_title":["LPS Administration Impacts Glial Immune Programs by Alternative Splicing."],"pmcid":["PMC8961644"],"funding_grant_id":["20-64-4701"],"pubmed_authors":["Dygalo NN","Shishkina GT","Babenko VN","Sukhareva EV","Lanshakov DA"],"additional_accession":[]},"is_claimable":false,"name":"LPS Administration Impacts Glial Immune Programs by Alternative Splicing.","description":"We performed transcriptome analysis in the hippocampus 24 h after lipopolysaccharide (LPS) administration. We observed glial-specific genes, comprised of two-thirds of all differentially expressed genes (DEGs). We found microglial DEGs that were the most numerous in LPS group. On the contrary, differential alternative splicing (DAS) analysis revealed the most numerous DAS events in astrocytes. Besides, we observed distinct major isoform switching in the <i>Ptbp1</i> gene, with skipping of exon 8 in LPS group. <i>Ptbp1</i> usually considered a pluripotency sustaining agent in brain embryonic development, according to the previous studies. Analyzing the splicing tune-up upon LPS exposure, we came to a supposition that the short <i>Ptbp1</i> isoform de-represses immune-specific response by <i>Ptbp1</i> adjusted splicing architecture. Additionally, the <i>Ptbp3</i> (<i>NOD1</i>) immune-specific splicing factor has apparently been de-repressed by the <i>Ptbp1</i> short isoform in glial cells. Notably, both the <i>Ptbp1</i> and <i>Ptbp3</i> genes express primarily in microglial/endothelial brain cells. We also report immune-related genes, altering their major isoforms upon LPS exposure. The results revealed immune modulating role of alternative splicing in brain.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2026-04-08T17:56:34.293Z","creation":"2025-04-06T21:53:09.021Z"},"accession":"S-EPMC8961644","cross_references":{"pubmed":["35204777"],"doi":["10.3390/biom12020277"]}}