biostudies-literatureZhang YKey Technologies Research and Development ProgramDepartment of Science and Technology of Sichuan Province165https://www.ebi.ac.uk/biostudies/studies/S-EPMC8961928biostudies-literatureUnknown20(1)<h4>Background</h4>Browning of white adipose tissue (WAT) is a particularly appealing target for therapeutics in the treatment of obesity and related metabolic diseases. Although small extracellular vesicles (sEVs) released from adipose tissue (sEVs-AT) have emerged as novel player that regulate systemic metabolism by connecting different organs, the role of specific contents in sEVs-AT played in WAT browning has not been clarified.<h4>Results</h4>We revealed Nucleophosmin3 (NPM3), which was mainly transferred by sEVs derived from brown adipose tissue (sEVs-BAT), was served as a batokine that could induce WAT browning by regulating the stability of PRDM16 mRNA. sEVs-BAT enhanced the expressions of browning related genes in 3T3-L1 preadipocytes and WAT while knocking down of NPM3 in BAT impJournal of nanobiotechnologyNucleophosmin3 carried by small extracellular vesicles contribute to white adipose tissue browning.PMC89619282017YFA01048002019YFS0312Yu MTian WZhang YDong JWu YfalseNucleophosmin3 carried by small extracellular vesicles contribute to white adipose tissue browning.<h4>Background</h4>Browning of white adipose tissue (WAT) is a particularly appealing target for therapeutics in the treatment of obesity and related metabolic diseases. Although small extracellular vesicles (sEVs) released from adipose tissue (sEVs-AT) have emerged as novel player that regulate systemic metabolism by connecting different organs, the role of specific contents in sEVs-AT played in WAT browning has not been clarified.<h4>Results</h4>We revealed Nucleophosmin3 (NPM3), which was mainly transferred by sEVs derived from brown adipose tissue (sEVs-BAT), was served as a batokine that could induce WAT browning by regulating the stability of PRDM16 mRNA. sEVs-BAT enhanced the expressions of browning related genes in 3T3-L1 preadipocytes and WAT while knocking down of NPM3 in BAT imp2022-01-01T00:00:00Z2022 Mar2025-04-04T19:10:21.918Z2025-04-04T19:10:21.918ZS-EPMC89619283534621310.1186/s12951-022-01381-1