{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ji SF"],"funding":["the National Natural Science Foundation of China"],"pagination":["13"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8962256"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(1)"],"pubmed_abstract":["Background
Large skin defects severely disrupt the overall skin structure and can irreversibly damage sweat glands (SG), thus impairing the skin's physiological function. This study aims to develop a stepwise reprogramming strategy to convert fibroblasts into SG lineages, which may provide a promising method to obtain desirable cell types for the functional repair and regeneration of damaged skin.Methods
The expression of the SG markers cytokeratin 5 (CK5), cytokeratin 10 (CK10), cytokeratin 18 (CK18), carcino-embryonic antigen (CEA), aquaporin 5 (AQP5) and α-smooth muscle actin (α-SMA) was assessed with quantitative PCR (qPCR), immunofluorescence and flow cytometry. Calcium activity analysis was conducted to test the function of induced SG-like cells (iSGCs). Mouse xenogra"],"journal":["Military Medical Research"],"pubmed_title":["Small molecules facilitate single factor-mediated sweat gland cell reprogramming."],"pmcid":["PMC8962256"],"funding_grant_id":["81871569"],"pubmed_authors":["Sun XY","Zhou LX","Li Y","Sun ZF","Chen HT","Xiang JB","Ji SF","Cui SY","Gao HH","Fu XB","Liu YQ"],"additional_accession":[]},"is_claimable":false,"name":"Small molecules facilitate single factor-mediated sweat gland cell reprogramming.","description":"Background
Large skin defects severely disrupt the overall skin structure and can irreversibly damage sweat glands (SG), thus impairing the skin's physiological function. This study aims to develop a stepwise reprogramming strategy to convert fibroblasts into SG lineages, which may provide a promising method to obtain desirable cell types for the functional repair and regeneration of damaged skin.Methods
The expression of the SG markers cytokeratin 5 (CK5), cytokeratin 10 (CK10), cytokeratin 18 (CK18), carcino-embryonic antigen (CEA), aquaporin 5 (AQP5) and α-smooth muscle actin (α-SMA) was assessed with quantitative PCR (qPCR), immunofluorescence and flow cytometry. Calcium activity analysis was conducted to test the function of induced SG-like cells (iSGCs). Mouse xenogra","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-06-20T04:51:38.309Z","creation":"2025-04-06T15:49:27.761Z"},"accession":"S-EPMC8962256","cross_references":{"pubmed":["35351192"],"doi":["10.1186/s40779-022-00372-5"]}}