{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hirano S"],"funding":["Takeda Science Foundation","Ministry of Education, Culture, Sports, Science and Technology","Japan Society for the Promotion of Science"],"pagination":["383"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8962430"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(2)"],"pubmed_abstract":["The purpose of this study was to develop a method to comprehensively determine the localization of apical and basolateral membrane proteins, using a combination of apical/basolateral membrane separation and accurate SWATH (<b>S</b>equential <b>W</b>indow <b>A</b>cquisition of all <b>TH</b>eoretical fragment ion spectra) proteomics. The SWATH analysis of basolateral and apical plasma membrane fractions in mouse liver quantified the protein expression of 1373 proteins. The basolateral/apical ratios of the protein expression levels were compared with the reported immunohistochemical localization for 41 model proteins (23 basolateral, 11 apical and 7 both membrane-localized proteins). Three groups were perfectly distinguished. Border lines to distinguish the apical-, both- and basolateral loca"],"journal":["Biomedicines"],"pubmed_title":["SWATH-Based Comprehensive Determination of the Localization of Apical and Basolateral Membrane Proteins Using Mouse Liver as a Model Tissue."],"pmcid":["PMC8962430"],"funding_grant_id":["N/A","KAKENHI: 20H05495","KAKENHI: 18KK0446","KAKENHI: 20H04690","KAKENHI: 20H03399"],"pubmed_authors":["Hirano S","Uchida Y","Goto R"],"additional_accession":[]},"is_claimable":false,"name":"SWATH-Based Comprehensive Determination of the Localization of Apical and Basolateral Membrane Proteins Using Mouse Liver as a Model Tissue.","description":"The purpose of this study was to develop a method to comprehensively determine the localization of apical and basolateral membrane proteins, using a combination of apical/basolateral membrane separation and accurate SWATH (<b>S</b>equential <b>W</b>indow <b>A</b>cquisition of all <b>TH</b>eoretical fragment ion spectra) proteomics. The SWATH analysis of basolateral and apical plasma membrane fractions in mouse liver quantified the protein expression of 1373 proteins. The basolateral/apical ratios of the protein expression levels were compared with the reported immunohistochemical localization for 41 model proteins (23 basolateral, 11 apical and 7 both membrane-localized proteins). Three groups were perfectly distinguished. Border lines to distinguish the apical-, both- and basolateral loca","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2026-04-08T17:56:23.731Z","creation":"2025-04-05T15:22:58.426Z"},"accession":"S-EPMC8962430","cross_references":{"pubmed":["35203592"],"doi":["10.3390/biomedicines10020383"]}}