biostudies-literatureUnknown14(2)Wang MNational Centre for the Replacement, Refinement and Reduction of Animals in ResearchNIBIB NIH HHSEuropean UnionBrigham Research InstituteNCI NIH HHSNational Institutes of HealthNIGMS NIH HHSNational Science FoundationThree-dimensional (3D) bioprinting has emerged as an enabling tool for various biomedical applications, such as tissue regeneration and tissue model engineering. To this end, the development of bioinks with multiple functions plays a crucial role in the applications of 3D bioprinting technologies. In this study, we propose a new bioink based on two immiscible aqueous phases of gelatin methacryloyl (GelMA) and dextran, further endowed with anti-bacterial and anti-inflammatory properties. This micropore-forming GelMA-dextran (PGelDex) bioink exhibited excellent printability with vat-polymerization, extrusion, and handheld bioprinting methods. The porous structure was confirmed after bioprinting, which promoted the spreading of the encapsulated cells, exhibiting the exceptional cytocompatibilBiofabricationhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC8962756biostudies-literatureA multifunctional micropore-forming bioink with enhanced anti-bacterial and anti-inflammatory properties.PMC8962756R01 EB028143NANC/K500318/1CBET-EBMS-123859R01 GM134036R21 EB025270R21 EB026175R00CA201603760921R00 CA201603Mu XLuo ZTang GKuang XZhao ZWang MLi WJiang ZLian LGuo JGhaemmaghami AMZhang YSFlores RSJapo JOfalseA multifunctional micropore-forming bioink with enhanced anti-bacterial and anti-inflammatory properties.Three-dimensional (3D) bioprinting has emerged as an enabling tool for various biomedical applications, such as tissue regeneration and tissue model engineering. To this end, the development of bioinks with multiple functions plays a crucial role in the applications of 3D bioprinting technologies. In this study, we propose a new bioink based on two immiscible aqueous phases of gelatin methacryloyl (GelMA) and dextran, further endowed with anti-bacterial and anti-inflammatory properties. This micropore-forming GelMA-dextran (PGelDex) bioink exhibited excellent printability with vat-polymerization, extrusion, and handheld bioprinting methods. The porous structure was confirmed after bioprinting, which promoted the spreading of the encapsulated cells, exhibiting the exceptional cytocompatibil2022-01-01T00:00:00Z2022 Mar2025-04-18T22:56:10.239Z2025-04-07T10:38:34.559ZS-EPMC89627563522688010.1088/1758-5090/ac5936