{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Frumm SM"],"funding":["NIDCR NIH HHS","National Institute of Diabetes and Digestive and Kidney Diseases","NIDDK NIH HHS","American Otological Society","NIDCD NIH HHS","National Institute on Deafness and Other Communication Disorders","National Institute of Dental and Craniofacial Research","NIH HHS"],"pagination":["315-330.e5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8962966"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(2)"],"pubmed_abstract":["The tympanic membrane (TM) is critical for hearing and requires continuous clearing of cellular debris, but little is known about homeostatic mechanisms in the TM epidermis. Using single-cell RNA sequencing, lineage tracing, whole-organ explant, and live-cell imaging, we show that homeostatic TM epidermis is distinct from other epidermal sites and has discrete proliferative zones with a three-dimensional hierarchy of multiple keratinocyte populations. TM stem cells reside in a discrete location of the superior TM and generate long-lived clones and committed progenitors (CPs). CP clones exhibit lateral migration, and their proliferative capacity is supported by Pdgfra+ fibroblasts, generating migratory but non-proliferative progeny. Single-cell sequencing of the human TM revealed similar ce"],"journal":["Cell stem cell"],"pubmed_title":["A Hierarchy of Proliferative and Migratory Keratinocytes Maintains the Tympanic Membrane."],"pmcid":["PMC8962966"],"funding_grant_id":["R01 DE029890","R01 DK118421","S10 OD021664","R01 DC018076"],"pubmed_authors":["Frumm SM","Tward AD","Yu SK","Lee KP","Byrnes LE","Chang J","Artichoker JA","Scaria SM","Sneddon JB"],"additional_accession":[]},"is_claimable":false,"name":"A Hierarchy of Proliferative and Migratory Keratinocytes Maintains the Tympanic Membrane.","description":"The tympanic membrane (TM) is critical for hearing and requires continuous clearing of cellular debris, but little is known about homeostatic mechanisms in the TM epidermis. Using single-cell RNA sequencing, lineage tracing, whole-organ explant, and live-cell imaging, we show that homeostatic TM epidermis is distinct from other epidermal sites and has discrete proliferative zones with a three-dimensional hierarchy of multiple keratinocyte populations. TM stem cells reside in a discrete location of the superior TM and generate long-lived clones and committed progenitors (CPs). CP clones exhibit lateral migration, and their proliferative capacity is supported by Pdgfra+ fibroblasts, generating migratory but non-proliferative progeny. Single-cell sequencing of the human TM revealed similar ce","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2026-05-30T15:02:41.041Z","creation":"2025-04-04T23:04:24.025Z"},"accession":"S-EPMC8962966","cross_references":{"pubmed":["33181078"],"doi":["10.1016/j.stem.2020.10.006"]}}