{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen Q"],"funding":["National Health and Medical Research Council","Australian Research Council"],"pagination":["813087"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8963460"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["Coronavirus disease 2019 (COVID-19) caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an acute respiratory disease with systemic complications. Therapeutic strategies for COVID-19, including repurposing (partially) developed drugs are urgently needed, regardless of the increasingly successful vaccination outcomes. We characterized two-dimensional (2D) and three-dimensional models (3D) to establish a physiologically relevant airway epithelial model with potential for investigating SARS-CoV-2 therapeutics. Human airway basal epithelial cells maintained in submerged 2D culture were used at low passage to retain the capacity to differentiate into ciliated, club, and goblet cells in both air-liquid interface culture (ALI) and airway organoid cultures, "],"journal":["Frontiers in pharmacology"],"pubmed_title":["ACE2 Expression in Organotypic Human Airway Epithelial Cultures and Airway Biopsies."],"pmcid":["PMC8963460"],"funding_grant_id":["IC170100016","1181637"],"pubmed_authors":["Chen Q","Baker ML","Gartner MJ","Xia YC","Subbarao K","Todd S","Clarke N","Langenbach S","Li M","Pharo EA","Williams SM","Stewart AG","Gao X","Ranganathan S"],"additional_accession":[]},"is_claimable":false,"name":"ACE2 Expression in Organotypic Human Airway Epithelial Cultures and Airway Biopsies.","description":"Coronavirus disease 2019 (COVID-19) caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an acute respiratory disease with systemic complications. Therapeutic strategies for COVID-19, including repurposing (partially) developed drugs are urgently needed, regardless of the increasingly successful vaccination outcomes. We characterized two-dimensional (2D) and three-dimensional models (3D) to establish a physiologically relevant airway epithelial model with potential for investigating SARS-CoV-2 therapeutics. Human airway basal epithelial cells maintained in submerged 2D culture were used at low passage to retain the capacity to differentiate into ciliated, club, and goblet cells in both air-liquid interface culture (ALI) and airway organoid cultures, ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-19T00:39:25.105Z","creation":"2025-04-07T11:41:23.486Z"},"accession":"S-EPMC8963460","cross_references":{"pubmed":["35359837"],"doi":["10.3389/fphar.2022.813087"]}}