biostudies-literatureChen QNational Health and Medical Research CouncilAustralian Research Council813087https://www.ebi.ac.uk/biostudies/studies/S-EPMC8963460biostudies-literatureUnknown13Coronavirus disease 2019 (COVID-19) caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an acute respiratory disease with systemic complications. Therapeutic strategies for COVID-19, including repurposing (partially) developed drugs are urgently needed, regardless of the increasingly successful vaccination outcomes. We characterized two-dimensional (2D) and three-dimensional models (3D) to establish a physiologically relevant airway epithelial model with potential for investigating SARS-CoV-2 therapeutics. Human airway basal epithelial cells maintained in submerged 2D culture were used at low passage to retain the capacity to differentiate into ciliated, club, and goblet cells in both air-liquid interface culture (ALI) and airway organoid cultures, Frontiers in pharmacologyACE2 Expression in Organotypic Human Airway Epithelial Cultures and Airway Biopsies.PMC8963460IC1701000161181637Chen QBaker MLGartner MJXia YCSubbarao KTodd SClarke NLangenbach SLi MPharo EAWilliams SMStewart AGGao XRanganathan SfalseACE2 Expression in Organotypic Human Airway Epithelial Cultures and Airway Biopsies.Coronavirus disease 2019 (COVID-19) caused by infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an acute respiratory disease with systemic complications. Therapeutic strategies for COVID-19, including repurposing (partially) developed drugs are urgently needed, regardless of the increasingly successful vaccination outcomes. We characterized two-dimensional (2D) and three-dimensional models (3D) to establish a physiologically relevant airway epithelial model with potential for investigating SARS-CoV-2 therapeutics. Human airway basal epithelial cells maintained in submerged 2D culture were used at low passage to retain the capacity to differentiate into ciliated, club, and goblet cells in both air-liquid interface culture (ALI) and airway organoid cultures, 2022-01-01T00:00:00Z20222025-04-19T00:39:25.105Z2025-04-07T11:41:23.486ZS-EPMC89634603535983710.3389/fphar.2022.813087