{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Wang H"],"funding":["Taishan Scholar Project of Shandong Province","National Natural Science Foundation of China"],"pubmed_abstract":["<b>Background:</b> The interaction between lysosomes and mitochondria includes not only mitophagy but also mitochondrion-lysosome contact (MLC) that enables the two organelles to exchange materials and information. In our study, we synthesised a biosensor with fluorescence characteristics that can image lysosomes for structured illumination microscopy and, in turn, examined morphological changes in mitochondria and the phenomenon of MLC under pathological conditions. <b>Methods:</b> After designing and synthesising the biosensor, dubbed CNN, we performed an assay with a Cell Counting Kit-8 to detect CNN's toxicity in relation to H9C2 cardiomyocytes. We next analysed the co-localisation of CNN and the commercial lysosomal probe LTG in cells, qualitatively analysed the imaging characteristic"],"journal":["Frontiers in pharmacology"],"pagination":["865173"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8965020"],"repository":["biostudies-literature"],"pubmed_title":["Lysosome-Targeted Biosensor for the Super-Resolution Imaging of Lysosome-Mitochondrion Interaction."],"pmcid":["PMC8965020"],"pubmed_authors":["Liu Y","Shao X","Wang B","Fang G","Cui Y","Hu M","Wang H","Chen H","Su Y","Dong B"],"additional_accession":[]},"is_claimable":false,"name":"Lysosome-Targeted Biosensor for the Super-Resolution Imaging of Lysosome-Mitochondrion Interaction.","description":"<b>Background:</b> The interaction between lysosomes and mitochondria includes not only mitophagy but also mitochondrion-lysosome contact (MLC) that enables the two organelles to exchange materials and information. In our study, we synthesised a biosensor with fluorescence characteristics that can image lysosomes for structured illumination microscopy and, in turn, examined morphological changes in mitochondria and the phenomenon of MLC under pathological conditions. <b>Methods:</b> After designing and synthesising the biosensor, dubbed CNN, we performed an assay with a Cell Counting Kit-8 to detect CNN's toxicity in relation to H9C2 cardiomyocytes. We next analysed the co-localisation of CNN and the commercial lysosomal probe LTG in cells, qualitatively analysed the imaging characteristic","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-04T07:35:11.528Z","creation":"2025-04-04T07:35:11.528Z"},"accession":"S-EPMC8965020","cross_references":{"pubmed":["35370649"],"doi":["10.3389/fphar.2022.865173"]}}