{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Goncalves MG"],"pubmed_abstract":["Brazil currently has the highest number of individuals infected with human T-lymphotropic virus 1- and 2- (HTLV-1 and HTLV-2) globally. At present, neither molecular protocols nor commercial assays are available for HTLV-1/-2 diagnosis or validated by the Brazilian Ministry of Health regulatory agency (ANVISA). We developed and validated two in-house multiplex quantitative real-time PCR for HTLV-1/-2 (mqPCR_HTLV) assays, targeting the <i>pol</i> and <i>tax</i> genes, for the simultaneous identification of HTLV-1, HTLV-2, and the albumin reference gene. The robustness of the assays was evaluated on two platforms using seven commercial master mix formulations. The reactions employed double plasmids (pHTLV1-Alb and pHTLV2-Alb) for the standard curve's construction and for expressing the detec"],"journal":["Frontiers in microbiology"],"pagination":["831594"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8965094"],"repository":["biostudies-literature"],"pubmed_title":["Development and Validation of Multiplex Quantitative Real-Time PCR Assays for Simultaneous Detection and Differentiation of HTLV-1 and HTLV-2, Using Different PCR Platforms and Reagent Brands."],"pmcid":["PMC8965094"],"pubmed_authors":["Campos KR","Fukasawa LO","Caterino-de-Araujo A","Higa FT","Goncalves MG"],"additional_accession":[]},"is_claimable":false,"name":"Development and Validation of Multiplex Quantitative Real-Time PCR Assays for Simultaneous Detection and Differentiation of HTLV-1 and HTLV-2, Using Different PCR Platforms and Reagent Brands.","description":"Brazil currently has the highest number of individuals infected with human T-lymphotropic virus 1- and 2- (HTLV-1 and HTLV-2) globally. At present, neither molecular protocols nor commercial assays are available for HTLV-1/-2 diagnosis or validated by the Brazilian Ministry of Health regulatory agency (ANVISA). We developed and validated two in-house multiplex quantitative real-time PCR for HTLV-1/-2 (mqPCR_HTLV) assays, targeting the <i>pol</i> and <i>tax</i> genes, for the simultaneous identification of HTLV-1, HTLV-2, and the albumin reference gene. The robustness of the assays was evaluated on two platforms using seven commercial master mix formulations. The reactions employed double plasmids (pHTLV1-Alb and pHTLV2-Alb) for the standard curve's construction and for expressing the detec","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-31T06:04:12.429Z","creation":"2025-02-19T01:55:12.05Z"},"accession":"S-EPMC8965094","cross_references":{"pubmed":["35369428"],"doi":["10.3389/fmicb.2022.831594"]}}