biostudies-literatureHardison RLEnvironmental Protection Agency (EPA) Office of Research and DevelopmentEPAIntramural EPA91-101https://www.ebi.ac.uk/biostudies/studies/S-EPMC8965596biostudies-literatureUnknown19(2)This study evaluated the efficacy of detergent-based surface cleaning methods against Murine Hepatitis Virus A59 (MHV) as a surrogate coronavirus for SARS-CoV-2. MHV (5% soil load in culture medium or simulated saliva) was inoculated onto four different high-touch materials [stainless steel (SS), Acrylonitrile Butadiene Styrene plastic (ABS), Formica, seat fabric (SF)]. Immediately and 2-hr post-inoculation, coupons were cleaned (damp wipe wiping) with and without pretreatment with detergent solution or 375 ppm hard water. Results identified that physical removal (no pretreatment) removed >2.3 log₁₀ MHV on ABS, SS, and Formica when surfaces were cleaned immediately. Pretreatment with detergent or hard water increased effectiveness over wet wiping 2-hr post-inoculation; pretreatmJournal of occupational and environmental hygieneEfficacy of detergent-based cleaning methods against coronavirus MHV-A59 on porous and non-porous surfaces.PMC8965596EPA999999EP-C-16-014EP-C-16-014/Task OrderBarriga DGhere JMHoward MWHardison RLNelson SWCalfee MWJames RRLee SDRyan SPFenton GAStewart MJfalseEfficacy of detergent-based cleaning methods against coronavirus MHV-A59 on porous and non-porous surfaces.This study evaluated the efficacy of detergent-based surface cleaning methods against Murine Hepatitis Virus A59 (MHV) as a surrogate coronavirus for SARS-CoV-2. MHV (5% soil load in culture medium or simulated saliva) was inoculated onto four different high-touch materials [stainless steel (SS), Acrylonitrile Butadiene Styrene plastic (ABS), Formica, seat fabric (SF)]. Immediately and 2-hr post-inoculation, coupons were cleaned (damp wipe wiping) with and without pretreatment with detergent solution or 375 ppm hard water. Results identified that physical removal (no pretreatment) removed >2.3 log₁₀ MHV on ABS, SS, and Formica when surfaces were cleaned immediately. Pretreatment with detergent or hard water increased effectiveness over wet wiping 2-hr post-inoculation; pretreatm2022-01-01T00:00:00Z2022 Feb2025-04-18T15:21:20.309Z2025-04-07T02:00:18.272ZS-EPMC89655963487835110.1080/15459624.2021.2015075