{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Brunner S"],"funding":["Laserlab-Europe","European Regional Development Fund"],"pagination":["1609971"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8966514"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27"],"pubmed_abstract":["The quantitative detection of radiation caused DNA double-strand breaks (DSB) by immunostained γ-H2AX foci using direct stochastic optical reconstruction microscopy (dSTORM) provides a deeper insight into the DNA repair process at nanoscale in a time-dependent manner. Glioblastoma (U251) cells were irradiated with 250 keV X-ray at 0, 2, 5, 8 Gy dose levels. Cell cycle phase distribution and apoptosis of U251 cells upon irradiation was assayed by flow cytometry. We studied the density, topology and volume of the γ-H2AX foci with 3D confocal microscopy and the dSTORM superresolution method. A pronounced increase in γ-H2AX foci and cluster density was detected by 3D confocal microscopy after 2 Gy, at 30 min postirradiation, but both returned to the control level at 24 h. Meanwhile, at 24 h a "],"journal":["Pathology oncology research : POR"],"pubmed_title":["Analysis of Ionizing Radiation Induced DNA Damage by Superresolution dSTORM Microscopy."],"pmcid":["PMC8966514"],"funding_grant_id":["871124","GINOP-2.3.6-15-2015-00001"],"pubmed_authors":["Varga D","Bozo R","Szabo ER","Erdelyi M","Hideghety K","Molnar R","Brunner S","Puskas LG","Gemes N","Tokes T","Szebeni GJ","Polanek R"],"additional_accession":[]},"is_claimable":false,"name":"Analysis of Ionizing Radiation Induced DNA Damage by Superresolution dSTORM Microscopy.","description":"The quantitative detection of radiation caused DNA double-strand breaks (DSB) by immunostained γ-H2AX foci using direct stochastic optical reconstruction microscopy (dSTORM) provides a deeper insight into the DNA repair process at nanoscale in a time-dependent manner. Glioblastoma (U251) cells were irradiated with 250 keV X-ray at 0, 2, 5, 8 Gy dose levels. Cell cycle phase distribution and apoptosis of U251 cells upon irradiation was assayed by flow cytometry. We studied the density, topology and volume of the γ-H2AX foci with 3D confocal microscopy and the dSTORM superresolution method. A pronounced increase in γ-H2AX foci and cluster density was detected by 3D confocal microscopy after 2 Gy, at 30 min postirradiation, but both returned to the control level at 24 h. Meanwhile, at 24 h a ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-08-23T03:04:54.182Z","creation":"2025-04-06T14:52:09.101Z"},"accession":"S-EPMC8966514","cross_references":{"pubmed":["35370480"],"doi":["10.3389/pore.2021.1609971"]}}