biostudies-literatureBrunner SLaserlab-EuropeEuropean Regional Development Fund1609971https://www.ebi.ac.uk/biostudies/studies/S-EPMC8966514biostudies-literatureUnknown27The quantitative detection of radiation caused DNA double-strand breaks (DSB) by immunostained γ-H2AX foci using direct stochastic optical reconstruction microscopy (dSTORM) provides a deeper insight into the DNA repair process at nanoscale in a time-dependent manner. Glioblastoma (U251) cells were irradiated with 250 keV X-ray at 0, 2, 5, 8 Gy dose levels. Cell cycle phase distribution and apoptosis of U251 cells upon irradiation was assayed by flow cytometry. We studied the density, topology and volume of the γ-H2AX foci with 3D confocal microscopy and the dSTORM superresolution method. A pronounced increase in γ-H2AX foci and cluster density was detected by 3D confocal microscopy after 2 Gy, at 30 min postirradiation, but both returned to the control level at 24 h. Meanwhile, at 24 h a Pathology oncology research : PORAnalysis of Ionizing Radiation Induced DNA Damage by Superresolution dSTORM Microscopy.PMC8966514871124GINOP-2.3.6-15-2015-00001Varga DBozo RSzabo ERErdelyi MHideghety KMolnar RBrunner SPuskas LGGemes NTokes TSzebeni GJPolanek RfalseAnalysis of Ionizing Radiation Induced DNA Damage by Superresolution dSTORM Microscopy.The quantitative detection of radiation caused DNA double-strand breaks (DSB) by immunostained γ-H2AX foci using direct stochastic optical reconstruction microscopy (dSTORM) provides a deeper insight into the DNA repair process at nanoscale in a time-dependent manner. Glioblastoma (U251) cells were irradiated with 250 keV X-ray at 0, 2, 5, 8 Gy dose levels. Cell cycle phase distribution and apoptosis of U251 cells upon irradiation was assayed by flow cytometry. We studied the density, topology and volume of the γ-H2AX foci with 3D confocal microscopy and the dSTORM superresolution method. A pronounced increase in γ-H2AX foci and cluster density was detected by 3D confocal microscopy after 2 Gy, at 30 min postirradiation, but both returned to the control level at 24 h. Meanwhile, at 24 h a 2021-01-01T00:00:00Z20212025-08-23T03:04:54.182Z2025-04-06T14:52:09.101ZS-EPMC89665143537048010.3389/pore.2021.1609971