{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang EY"],"funding":["NCI NIH HHS","NIH HHS"],"pagination":["100172"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8967185"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2(2)"],"pubmed_abstract":["Autoantibodies that recognize extracellular proteins (the exoproteome) exert potent biological effects but are challenging to detect. Here, we developed rapid extracellular antigen profiling (REAP), a high-throughput technique for the comprehensive discovery of exoproteome-targeting autoantibodies. Patient samples are applied to a genetically barcoded yeast surface display library containing 2,688 human extracellular proteins. Antibody-coated yeast are isolated, and sequencing of barcodes is used to identify displayed antigens. To benchmark REAP's performance, we screened 77 patients with autoimmune polyglandular syndrome type 1 (APS-1). REAP sensitively and specifically detected both known and previously unidentified autoantibodies in APS-1. We further screened 106 patients with systemic "],"journal":["Cell reports methods"],"pubmed_title":["High-throughput identification of autoantibodies that target the human exoproteome."],"pmcid":["PMC8967185"],"funding_grant_id":["DP5 OD023088","P30 CA016359"],"pubmed_authors":["Schmitt MM","Meffre E","Gonzalez-Hernandez JA","Dong MX","Koumpouras F","Dai Y","Wang EY","Rosen CE","Yang Y","Hinchcliff M","Ring AM","Ferre EMN","Lionakis MS","Liu F"],"additional_accession":[]},"is_claimable":false,"name":"High-throughput identification of autoantibodies that target the human exoproteome.","description":"Autoantibodies that recognize extracellular proteins (the exoproteome) exert potent biological effects but are challenging to detect. Here, we developed rapid extracellular antigen profiling (REAP), a high-throughput technique for the comprehensive discovery of exoproteome-targeting autoantibodies. Patient samples are applied to a genetically barcoded yeast surface display library containing 2,688 human extracellular proteins. Antibody-coated yeast are isolated, and sequencing of barcodes is used to identify displayed antigens. To benchmark REAP's performance, we screened 77 patients with autoimmune polyglandular syndrome type 1 (APS-1). REAP sensitively and specifically detected both known and previously unidentified autoantibodies in APS-1. We further screened 106 patients with systemic ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Feb","modification":"2026-05-31T11:12:46.535Z","creation":"2024-11-20T15:47:12.315Z"},"accession":"S-EPMC8967185","cross_references":{"pubmed":["35360706"],"doi":["10.1016/j.crmeth.2022.100172"]}}