{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kang D"],"funding":["Natural Sciences and Engineering Research Council of Canada","Nanyang Technological University","Basic Energy Sciences","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["13346-13352"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8968159"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["37(45)"],"pubmed_abstract":["Using a surface forces apparatus (SFA), we have studied the nanomechanical behavior of short single-stranded and partially and fully double-stranded DNA molecules attached via one end to a self-assembled monolayer on a gold surface. Our results confirm the previously proposed \"mushroom-like\" polymer structure for surface-attached, single-stranded DNA at low packing density and a \"brush-like\" structure for the same construct at higher density. At low density we observe a transition to \"rigid rod\" behavior upon addition of DNA complementary to the surface-attached single strand as the fraction of molecules that are double-stranded increases, with a concomitant increase in the SFA-observed thickness of the monolayer and the characteristic length of the observed repulsive forces. At higher densities, in contrast, this transition is effectively eliminated, presumably because the single-stranded state is already extended in its \"brush\" state. Taken together, these studies offer insights into the structure and physics of surface-attached short DNAs, providing new guidance for the rational design of DNA-modified functional surfaces."],"journal":["Langmuir : the ACS journal of surfaces and colloids"],"pubmed_title":["Nanometer-Scale Force Profiles of Short Single- and Double-Stranded DNA Molecules on a Gold Surface Measured Using a Surface Forces Apparatus."],"pmcid":["PMC8968159"],"funding_grant_id":["RO1GM118560","M4082049.070","R01 GM118560"],"pubmed_authors":["Israelachvili J","Plaxco KW","Huang J","Kang D","Zeng H","Yu J","Tirrell M","Xia F"],"additional_accession":[]},"is_claimable":false,"name":"Nanometer-Scale Force Profiles of Short Single- and Double-Stranded DNA Molecules on a Gold Surface Measured Using a Surface Forces Apparatus.","description":"Using a surface forces apparatus (SFA), we have studied the nanomechanical behavior of short single-stranded and partially and fully double-stranded DNA molecules attached via one end to a self-assembled monolayer on a gold surface. Our results confirm the previously proposed \"mushroom-like\" polymer structure for surface-attached, single-stranded DNA at low packing density and a \"brush-like\" structure for the same construct at higher density. At low density we observe a transition to \"rigid rod\" behavior upon addition of DNA complementary to the surface-attached single strand as the fraction of molecules that are double-stranded increases, with a concomitant increase in the SFA-observed thickness of the monolayer and the characteristic length of the observed repulsive forces. At higher densities, in contrast, this transition is effectively eliminated, presumably because the single-stranded state is already extended in its \"brush\" state. Taken together, these studies offer insights into the structure and physics of surface-attached short DNAs, providing new guidance for the rational design of DNA-modified functional surfaces.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2025-04-27T01:08:52.436Z","creation":"2025-04-06T18:07:22.423Z"},"accession":"S-EPMC8968159","cross_references":{"pubmed":["34730362"],"doi":["10.1021/acs.langmuir.1c01966"]}}