biostudies-literatureUnknown13Zhang ZThe species <i>Brassica rapa</i> includes several important vegetable crops. The draft reference genome of <i>B. rapa</i> ssp. <i>pekinensis</i> was completed in 2011, and it has since been updated twice. The pangenome with structural variations of 18 <i>B. rapa</i> accessions was published in 2021. Although extensive genomic analysis has been conducted on <i>B. rapa</i>, a comprehensive genome annotation including gene structure, alternative splicing (AS) events, and non-coding genes is still lacking. Therefore, we used the Pacific Biosciences (PacBio) single-molecular long-read technology to improve gene models and produced the annotated genome version 3.5. In total, we obtained 753,041 full-length non-chimeric (FLNC) reads and collapsed these into 92,810 non-redundant consensus isoformsFrontiers in plant science841618https://www.ebi.ac.uk/biostudies/studies/S-EPMC8968949biostudies-literatureImproved Reference Genome Annotation of <i>Brassica rapa</i> by Pacific Biosciences RNA Sequencing.PMC8968949Xi XWu JLi YLin RGuo JZhang ZLiang JWang XCai XfalseImproved Reference Genome Annotation of <i>Brassica rapa</i> by Pacific Biosciences RNA Sequencing.The species <i>Brassica rapa</i> includes several important vegetable crops. The draft reference genome of <i>B. rapa</i> ssp. <i>pekinensis</i> was completed in 2011, and it has since been updated twice. The pangenome with structural variations of 18 <i>B. rapa</i> accessions was published in 2021. Although extensive genomic analysis has been conducted on <i>B. rapa</i>, a comprehensive genome annotation including gene structure, alternative splicing (AS) events, and non-coding genes is still lacking. Therefore, we used the Pacific Biosciences (PacBio) single-molecular long-read technology to improve gene models and produced the annotated genome version 3.5. In total, we obtained 753,041 full-length non-chimeric (FLNC) reads and collapsed these into 92,810 non-redundant consensus isoforms2022-01-01T00:00:00Z20222026-04-08T17:47:04.893Z2025-02-19T01:55:11.914ZS-EPMC89689493537116810.3389/fpls.2022.841618