{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Garde A"],"funding":["NCCIH NIH HHS","NIA NIH HHS","NIDDK NIH HHS","NIH HHS","NIGMS NIH HHS"],"pagination":["732-749.e7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8969095"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["57(6)"],"pubmed_abstract":["Invasive cells use transient, energy-consuming protrusions to breach basement membrane (BM) barriers. Using the ATP sensor PercevalHR during anchor cell (AC) invasion in Caenorhabditis elegans, we show that BM invasion is accompanied by an ATP burst from mitochondria at the invasive front. RNAi screening and visualization of a glucose biosensor identified two glucose transporters, FGT-1 and FGT-2, which bathe invasive front mitochondria with glucose and facilitate the ATP burst to form protrusions. FGT-1 localizes at high levels along the invasive membrane, while FGT-2 is adaptive, enriching most strongly during BM breaching and when FGT-1 is absent. Cytosolic glycolytic enzymes that process glucose for mitochondrial ATP production cluster with invasive front mitochondria and promote higher mitochondrial membrane potential and ATP levels. Finally, we show that UNC-6 (netrin), which polarizes invasive protrusions, also orients FGT-1. These studies reveal a robust and integrated energy acquisition, processing, and delivery network that powers BM breaching."],"journal":["Developmental cell"],"pubmed_title":["Localized glucose import, glycolytic processing, and mitochondria generate a focused ATP burst to power basement-membrane invasion."],"pmcid":["PMC8969095"],"funding_grant_id":["R21 OD028766","R01 AG045183","R01 AT009050","R35 GM118049","DP1 DK113644","P40 OD010440"],"pubmed_authors":["Mutlu AS","Wang MC","Sherwood DR","Chi Q","Kenny IW","Garde A","Kelley LC"],"additional_accession":[]},"is_claimable":false,"name":"Localized glucose import, glycolytic processing, and mitochondria generate a focused ATP burst to power basement-membrane invasion.","description":"Invasive cells use transient, energy-consuming protrusions to breach basement membrane (BM) barriers. Using the ATP sensor PercevalHR during anchor cell (AC) invasion in Caenorhabditis elegans, we show that BM invasion is accompanied by an ATP burst from mitochondria at the invasive front. RNAi screening and visualization of a glucose biosensor identified two glucose transporters, FGT-1 and FGT-2, which bathe invasive front mitochondria with glucose and facilitate the ATP burst to form protrusions. FGT-1 localizes at high levels along the invasive membrane, while FGT-2 is adaptive, enriching most strongly during BM breaching and when FGT-1 is absent. Cytosolic glycolytic enzymes that process glucose for mitochondrial ATP production cluster with invasive front mitochondria and promote higher mitochondrial membrane potential and ATP levels. Finally, we show that UNC-6 (netrin), which polarizes invasive protrusions, also orients FGT-1. These studies reveal a robust and integrated energy acquisition, processing, and delivery network that powers BM breaching.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-21T14:19:16.982Z","creation":"2025-04-21T14:19:16.982Z"},"accession":"S-EPMC8969095","cross_references":{"pubmed":["35316617"],"doi":["10.1016/j.devcel.2022.02.019"]}}