biostudies-literatureUrsing JFCTSvenska LäkaresällskapetStockholm County CouncilAnthony Cerami and Ann Dunne Foundation for World Health1005-1011https://www.ebi.ac.uk/biostudies/studies/S-EPMC8969533biostudies-literatureUnknown77(4)<h4>Background</h4>Plasmodium falciparum strains that are resistant to standard-dose chloroquine can be treated by higher chloroquine concentrations maintained for a longer time in vivo.<h4>Objectives</h4>To determine the relative importance of chloroquine concentrations versus exposure time for elimination of chloroquine-susceptible and -resistant P. falciparum in vitro.<h4>Methods</h4>Chloroquine-susceptible (3D7) and -resistant (FCR3) strains were exposed in vitro to 1, 2, 4, 8, 16 or 32 times their respective 90% inhibitory chloroquine concentrations for 3, 5, 7 or 14 days and then followed until recrudescence, or not, by 42 days after the end of exposure.<h4>Results</h4>Exposure to chloroquine appeared to eliminate susceptible and resistant parasites, leaving small pyknotic apparentlyThe Journal of antimicrobial chemotherapyChloroquine-susceptible and -resistant Plasmodium falciparum strains survive high chloroquine concentrations by becoming dormant but are eliminated by prolonged exposure.PMC8969533UIDP/50026/2020UIDB/50026/20202016059720102011Ghanchi NKUrsing JVeiga MIKofoed PEAydin-Schmidt BCalcada CJohns RRombo LfalseChloroquine-susceptible and -resistant Plasmodium falciparum strains survive high chloroquine concentrations by becoming dormant but are eliminated by prolonged exposure.<h4>Background</h4>Plasmodium falciparum strains that are resistant to standard-dose chloroquine can be treated by higher chloroquine concentrations maintained for a longer time in vivo.<h4>Objectives</h4>To determine the relative importance of chloroquine concentrations versus exposure time for elimination of chloroquine-susceptible and -resistant P. falciparum in vitro.<h4>Methods</h4>Chloroquine-susceptible (3D7) and -resistant (FCR3) strains were exposed in vitro to 1, 2, 4, 8, 16 or 32 times their respective 90% inhibitory chloroquine concentrations for 3, 5, 7 or 14 days and then followed until recrudescence, or not, by 42 days after the end of exposure.<h4>Results</h4>Exposure to chloroquine appeared to eliminate susceptible and resistant parasites, leaving small pyknotic apparently2022-01-01T00:00:00Z2022 Mar2025-04-26T15:18:38.823Z2025-04-06T14:50:56.113ZS-EPMC89695333513707210.1093/jac/dkac008