{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rybashlykov D"],"funding":["Российский Фонд Фундаментальных Исследований","Fondation Leducq"],"pagination":["e0259174"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8970595"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(3)"],"pubmed_abstract":["Fluorescent imaging with voltage- or calcium-sensitive dyes, known as optical mapping, is one of the indispensable modern techniques to study cardiac or neural electrophysiology, unsurpassed by temporal and spatial resolution. High-speed CMOS cameras capable of optical registration of action potential propagation are in general very costly. We present a complete solution priced below US$1,000 (including camera and lens) at the moment of publication with an open-source image acquisition and processing software. We demonstrate that the iDS UI-3130CP rev.2 camera we used in this study is capable of 200x200 977 frames per second (FPS) action potential recordings from rodent hearts, with the signal-to-noise-ratio of a conditioned signal of 16 ± 10. A comparison with a specialized MiCAM Ultimate-L camera has shown that signal-to-noise ratio (SNR) while lower is sufficient for accurate measurements of AP waveform, conduction velocity (± 0.04 m/s) and action potential duration (± 7ms) in mouse and rat hearts. We used 4-aminopyridine to prolong the action potential duration in mouse heart, thus demonstrating that the proposed system is adequate for pharmacological studies."],"journal":["PloS one"],"pubmed_title":["Open-source low-cost cardiac optical mapping system."],"pmcid":["PMC8970595"],"funding_grant_id":["19-29-04111","project RHYTHM"],"pubmed_authors":["Rybashlykov D","Efimov IR","Syunyaev R","Lin Z","Brennan J"],"additional_accession":[]},"is_claimable":false,"name":"Open-source low-cost cardiac optical mapping system.","description":"Fluorescent imaging with voltage- or calcium-sensitive dyes, known as optical mapping, is one of the indispensable modern techniques to study cardiac or neural electrophysiology, unsurpassed by temporal and spatial resolution. High-speed CMOS cameras capable of optical registration of action potential propagation are in general very costly. We present a complete solution priced below US$1,000 (including camera and lens) at the moment of publication with an open-source image acquisition and processing software. We demonstrate that the iDS UI-3130CP rev.2 camera we used in this study is capable of 200x200 977 frames per second (FPS) action potential recordings from rodent hearts, with the signal-to-noise-ratio of a conditioned signal of 16 ± 10. A comparison with a specialized MiCAM Ultimate-L camera has shown that signal-to-noise ratio (SNR) while lower is sufficient for accurate measurements of AP waveform, conduction velocity (± 0.04 m/s) and action potential duration (± 7ms) in mouse and rat hearts. We used 4-aminopyridine to prolong the action potential duration in mouse heart, thus demonstrating that the proposed system is adequate for pharmacological studies.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-19T13:00:09.412Z","creation":"2025-04-19T13:00:09.412Z"},"accession":"S-EPMC8970595","cross_references":{"pubmed":["35358183"],"doi":["10.1371/journal.pone.0259174"]}}