biostudies-literatureUnknown10Zhang J<h4>Background</h4>Macrolides have been widely used to treat moderate-to-severe acne for more than 50 years. However, the prevalent antibiotic resistance of Propionibacterium acnes, along with the absence of clinically available resistance tests, has made macrolide misuse a frequent occurrence around the globe, with serious consequences.<h4>Objective</h4>We developed <i>Cutibacterium acnes quantitative PCR</i> (qPCR)-based antibiotics resistance assay (ACQUIRE) to enable fast and accurate detection of <i>C. acnes</i> macrolide resistance in clinical settings, representing an opportunity to administer antibiotics more wisely and improve the quality of care.<h4>Methods</h4>A cross-sectional observational study (<i>n</i> = 915) was conducted to probe into the macrolide resistance of <i>C. acnes</i> in patients with acne.<h4>Results</h4>The high sensitivity of ACQUIRE enabled us to reveal a much higher <i>C. acnes</i> 23S recombinant DNA (rDNA) point mutation rate (52%) and thus a higher macrolide resistance (75.5%) compared to previous reports. Carriage of ermX gene was discovered on 472 (53%) subjects, which concurs with previous studies.<h4>Conclusion</h4>The macrolide resistance of <i>C. acnes</i> is much higher than previously reported. Integrating ACQUIRE into acne treatment modalities may eliminate macrolide misuse and achieve better clinical improvements.Frontiers in public health787299https://www.ebi.ac.uk/biostudies/studies/S-EPMC8971513biostudies-literature<i>C. acnes</i> qPCR-Based Antibiotics Resistance Assay (ACQUIRE) Reveals Widespread Macrolide Resistance in Acne Patients and Can Eliminate Macrolide Misuse in Acne Treatment.PMC8971513Zhang JKang SChen LYu FMa XZhu FHan YAli CCZhang TLi ZChen YFu KShi JZhou HGao SXu Yfalse<i>C. acnes</i> qPCR-Based Antibiotics Resistance Assay (ACQUIRE) Reveals Widespread Macrolide Resistance in Acne Patients and Can Eliminate Macrolide Misuse in Acne Treatment.<h4>Background</h4>Macrolides have been widely used to treat moderate-to-severe acne for more than 50 years. However, the prevalent antibiotic resistance of Propionibacterium acnes, along with the absence of clinically available resistance tests, has made macrolide misuse a frequent occurrence around the globe, with serious consequences.<h4>Objective</h4>We developed <i>Cutibacterium acnes quantitative PCR</i> (qPCR)-based antibiotics resistance assay (ACQUIRE) to enable fast and accurate detection of <i>C. acnes</i> macrolide resistance in clinical settings, representing an opportunity to administer antibiotics more wisely and improve the quality of care.<h4>Methods</h4>A cross-sectional observational study (<i>n</i> = 915) was conducted to probe into the macrolide resistance of <i>C. acnes</i> in patients with acne.<h4>Results</h4>The high sensitivity of ACQUIRE enabled us to reveal a much higher <i>C. acnes</i> 23S recombinant DNA (rDNA) point mutation rate (52%) and thus a higher macrolide resistance (75.5%) compared to previous reports. Carriage of ermX gene was discovered on 472 (53%) subjects, which concurs with previous studies.<h4>Conclusion</h4>The macrolide resistance of <i>C. acnes</i> is much higher than previously reported. Integrating ACQUIRE into acne treatment modalities may eliminate macrolide misuse and achieve better clinical improvements.2022-01-01T00:00:00Z20222024-11-09T07:54:36.465Z2024-11-09T07:54:36.465ZS-EPMC89715133537223110.3389/fpubh.2022.787299