{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gao L"],"funding":["European Molecular Biology Organization","Österreichische Forschungsförderungsgesellschaft","Deutsche Forschungsgemeinschaft","European Research Council","Ministerio de Ciencia e Innovación","Joachim Herz Stiftung","National Institutes of Health","NIGMS NIH HHS"],"pagination":["5614-5628"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8972266"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["144(12)"],"pubmed_abstract":["Photoswitchable reagents are powerful tools for high-precision studies in cell biology. When these reagents are globally administered yet locally photoactivated in two-dimensional (2D) cell cultures, they can exert micron- and millisecond-scale biological control. This gives them great potential for use in biologically more relevant three-dimensional (3D) models and <i>in vivo</i>, particularly for studying systems with inherent spatiotemporal complexity, such as the cytoskeleton. However, due to a combination of photoswitch isomerization under typical imaging conditions, metabolic liabilities, and insufficient water solubility at effective concentrations, the <i>in vivo</i> potential of photoswitchable reagents addressing cytosolic protein targets remains largely unrealized. Here, we opti"],"journal":["Journal of the American Chemical Society"],"pubmed_title":["<i>In Vivo</i> Photocontrol of Microtubule Dynamics and Integrity, Migration and Mitosis, by the Potent GFP-Imaging-Compatible Photoswitchable Reagents SBTubA4P and SBTub2M."],"pmcid":["PMC8972266"],"funding_grant_id":["1R01GM126029","7940628","ALTF 261-2019","RTI2018-096948-B-100","426018126","239283807","R01 GM126029","400324123","201269156"],"pubmed_authors":["Varady A","Taylor JA","Terni B","Bausch AR","Wranik M","Weinert T","Akhmanova A","Meiring JCM","Velasco CD","Standfuss J","Thorn-Seshold O","Heise C","Gao L","Distel M","Thorn-Seshold J","Ruider IE","Steinmetz MO","Cabernard CC","Llobet A"],"additional_accession":[]},"is_claimable":false,"name":"<i>In Vivo</i> Photocontrol of Microtubule Dynamics and Integrity, Migration and Mitosis, by the Potent GFP-Imaging-Compatible Photoswitchable Reagents SBTubA4P and SBTub2M.","description":"Photoswitchable reagents are powerful tools for high-precision studies in cell biology. When these reagents are globally administered yet locally photoactivated in two-dimensional (2D) cell cultures, they can exert micron- and millisecond-scale biological control. This gives them great potential for use in biologically more relevant three-dimensional (3D) models and <i>in vivo</i>, particularly for studying systems with inherent spatiotemporal complexity, such as the cytoskeleton. However, due to a combination of photoswitch isomerization under typical imaging conditions, metabolic liabilities, and insufficient water solubility at effective concentrations, the <i>in vivo</i> potential of photoswitchable reagents addressing cytosolic protein targets remains largely unrealized. Here, we opti","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-05-31T01:05:06.894Z","creation":"2025-04-04T11:31:35.074Z"},"accession":"S-EPMC8972266","cross_references":{"pubmed":["35290733"],"doi":["10.1021/jacs.2c01020"]}}