biostudies-literatureGao LEuropean Molecular Biology OrganizationÖsterreichische ForschungsförderungsgesellschaftDeutsche ForschungsgemeinschaftEuropean Research CouncilMinisterio de Ciencia e InnovaciónJoachim Herz StiftungNational Institutes of HealthNIGMS NIH HHS5614-5628https://www.ebi.ac.uk/biostudies/studies/S-EPMC8972266biostudies-literatureUnknown144(12)Photoswitchable reagents are powerful tools for high-precision studies in cell biology. When these reagents are globally administered yet locally photoactivated in two-dimensional (2D) cell cultures, they can exert micron- and millisecond-scale biological control. This gives them great potential for use in biologically more relevant three-dimensional (3D) models and <i>in vivo</i>, particularly for studying systems with inherent spatiotemporal complexity, such as the cytoskeleton. However, due to a combination of photoswitch isomerization under typical imaging conditions, metabolic liabilities, and insufficient water solubility at effective concentrations, the <i>in vivo</i> potential of photoswitchable reagents addressing cytosolic protein targets remains largely unrealized. Here, we optiJournal of the American Chemical Society<i>In Vivo</i> Photocontrol of Microtubule Dynamics and Integrity, Migration and Mitosis, by the Potent GFP-Imaging-Compatible Photoswitchable Reagents SBTubA4P and SBTub2M.PMC89722661R01GM1260297940628ALTF 261-2019RTI2018-096948-B-100426018126239283807R01 GM126029400324123201269156Varady ATaylor JATerni BBausch ARWranik MWeinert TAkhmanova AMeiring JCMVelasco CDStandfuss JThorn-Seshold OHeise CGao LDistel MThorn-Seshold JRuider IESteinmetz MOCabernard CCLlobet Afalse<i>In Vivo</i> Photocontrol of Microtubule Dynamics and Integrity, Migration and Mitosis, by the Potent GFP-Imaging-Compatible Photoswitchable Reagents SBTubA4P and SBTub2M.Photoswitchable reagents are powerful tools for high-precision studies in cell biology. When these reagents are globally administered yet locally photoactivated in two-dimensional (2D) cell cultures, they can exert micron- and millisecond-scale biological control. This gives them great potential for use in biologically more relevant three-dimensional (3D) models and <i>in vivo</i>, particularly for studying systems with inherent spatiotemporal complexity, such as the cytoskeleton. However, due to a combination of photoswitch isomerization under typical imaging conditions, metabolic liabilities, and insufficient water solubility at effective concentrations, the <i>in vivo</i> potential of photoswitchable reagents addressing cytosolic protein targets remains largely unrealized. Here, we opti2022-01-01T00:00:00Z2022 Mar2026-05-31T01:05:06.894Z2025-04-04T11:31:35.074ZS-EPMC89722663529073310.1021/jacs.2c01020