{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Malla P"],"funding":["Ministry of Science and Technology, Taiwan","Chang Gung University","Chang Gung Memorial Hospital, Linkou"],"pagination":["168"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8973645"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["189(4)"],"pubmed_abstract":["The rapid spread of the novel human coronavirus 2019 (COVID-19) and its morbidity have created an urgent need for rapid and sensitive diagnostics. The real-time polymerase chain reaction is the gold standard for detecting the coronavirus in various types of biological specimens. However, this technique is time consuming, labor intensive, and expensive. Screen-printed electrodes (SPEs) can be used as point-of-care devices because of their low cost, sensitivity, selectivity, and ability to be miniaturized. The ability to detect the spike protein of COVID-19 in serum, urine, and saliva was developed using SPE aided by magnetic beads (MBs) and a portable potentiostat. The antibody-peroxidase-loaded MBs were the captured and catalytic units for the electrochemical assays. The MBs enable simple washing and homogenous deposition on the working electrode using a magnet. The assembly of the immunological MBs and the electrochemical system increases the measuring sensitivity and speed. The physical and electrochemical properties of the layer-by-layer modified MBs were systematically characterized. The performance of these immunosensors was evaluated using spike protein in the range 3.12-200 ng mL<sup>-1</sup>. We achieved a limit of detection of 0.20, 0.31, and 0.54 ng mL<sup>-1</sup> in human saliva, urine, and serum, respectively. A facile electrochemical method to detect COVID-19 spike protein was developed for quick point-of-care testing."],"journal":["Mikrochimica acta"],"pubmed_title":["Voltammetric biosensor for coronavirus spike protein using magnetic bead and screen-printed electrode for point-of-care diagnostics."],"pmcid":["PMC8973645"],"funding_grant_id":["BMRP 758","110-2221-E-182-024","2H0073"],"pubmed_authors":["Liu CH","Wu WC","Sreearunothai P","Malla P","Liao HP"],"additional_accession":[]},"is_claimable":false,"name":"Voltammetric biosensor for coronavirus spike protein using magnetic bead and screen-printed electrode for point-of-care diagnostics.","description":"The rapid spread of the novel human coronavirus 2019 (COVID-19) and its morbidity have created an urgent need for rapid and sensitive diagnostics. The real-time polymerase chain reaction is the gold standard for detecting the coronavirus in various types of biological specimens. However, this technique is time consuming, labor intensive, and expensive. Screen-printed electrodes (SPEs) can be used as point-of-care devices because of their low cost, sensitivity, selectivity, and ability to be miniaturized. The ability to detect the spike protein of COVID-19 in serum, urine, and saliva was developed using SPE aided by magnetic beads (MBs) and a portable potentiostat. The antibody-peroxidase-loaded MBs were the captured and catalytic units for the electrochemical assays. The MBs enable simple washing and homogenous deposition on the working electrode using a magnet. The assembly of the immunological MBs and the electrochemical system increases the measuring sensitivity and speed. The physical and electrochemical properties of the layer-by-layer modified MBs were systematically characterized. The performance of these immunosensors was evaluated using spike protein in the range 3.12-200 ng mL<sup>-1</sup>. We achieved a limit of detection of 0.20, 0.31, and 0.54 ng mL<sup>-1</sup> in human saliva, urine, and serum, respectively. A facile electrochemical method to detect COVID-19 spike protein was developed for quick point-of-care testing.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-04T23:34:39.697Z","creation":"2025-04-04T23:34:39.697Z"},"accession":"S-EPMC8973645","cross_references":{"pubmed":["35362759"],"doi":["10.1007/s00604-022-05288-4"]}}