{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jiang Y"],"funding":["Health Commission of Sichuan Province (Universal Application Project"],"pagination":["4688-4701"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8973977"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(3)"],"pubmed_abstract":["Alcoholic liver disease (ALD) denotes a series of liver diseases caused by ethanol. Recently, immune-related genes (IRGs) play increasingly crucial role in diseases. However, it's unclear the role of IRGs in ALD. Bioinformatic analysis was used to discern the core immune-related differential genes (IRDGs) in the present study. Subsequently, Cell Counting Kit-8 say, oil red O staining, and triglyceride detection were employed to explore optimal experimental conditions of establishing hepatocellular models of early ALD. Ultimately, real-time reverse transcription-PCR and immunohistochemistry/immunocytochemistry methods were adopted to verify the expressions of mRNA and proteins of core IRDGs, respectively. C-X-C Motif Chemokine Ligand 1 (<i>Cxcl1</i>) and <i>Cxcl6</i> were regarded as core I"],"journal":["Bioengineered"],"pubmed_title":["Ethanol promoting the upregulation of C-X-C Motif Chemokine Ligand 1（CXCL1） and C-X-C Motif Chemokine Ligand 6（CXCL6） in models of early alcoholic liver disease."],"pmcid":["PMC8973977"],"funding_grant_id":["20PJ144"],"pubmed_authors":["Jiang Y","Zeng C","Li Y","Xi Y","Zhang D","Zuo Z","Fan J","Guo Y","Tao H"],"additional_accession":[]},"is_claimable":false,"name":"Ethanol promoting the upregulation of C-X-C Motif Chemokine Ligand 1（CXCL1） and C-X-C Motif Chemokine Ligand 6（CXCL6） in models of early alcoholic liver disease.","description":"Alcoholic liver disease (ALD) denotes a series of liver diseases caused by ethanol. Recently, immune-related genes (IRGs) play increasingly crucial role in diseases. However, it's unclear the role of IRGs in ALD. Bioinformatic analysis was used to discern the core immune-related differential genes (IRDGs) in the present study. Subsequently, Cell Counting Kit-8 say, oil red O staining, and triglyceride detection were employed to explore optimal experimental conditions of establishing hepatocellular models of early ALD. Ultimately, real-time reverse transcription-PCR and immunohistochemistry/immunocytochemistry methods were adopted to verify the expressions of mRNA and proteins of core IRDGs, respectively. C-X-C Motif Chemokine Ligand 1 (<i>Cxcl1</i>) and <i>Cxcl6</i> were regarded as core I","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-19T09:50:36.856Z","creation":"2025-04-19T09:50:36.856Z"},"accession":"S-EPMC8973977","cross_references":{"pubmed":["35156518"],"doi":["10.1080/21655979.2022.2030557"]}}