{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Song W"],"funding":["Dalian Municipal Science and Technology Bureau"],"pagination":["e0266332"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8974964"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(4)"],"pubmed_abstract":["<h4>Objectives</h4>To assess the efficacy of Y-chromosome mini-STR-based next-generation sequencing (NGS) for non-invasive prenatal paternity testing (NIPPT).<h4>Methods</h4>DNA was extracted from the plasma of 24 pregnant women, and cell-free fetal DNA (cffDNA) haplotyping was performed at 12 Y-chromosome mini-STR loci using the Illumina NextSeq 500 system. The cffDNA haplotype was validated by the paternal haplotype. Subsequentlly, the paternity testing parameters were attributed to each case quantitatively.<h4>Results</h4>The biological relationship between the alleged fathers and infants in all 24 family cases were confirmed by capillary electrophoresis (CE). The Y-chromosome mini-STR haplotypes of all 14 male cffDNA were obtained by NGS without any missing loci. The alleles of cffDNA "],"journal":["PloS one"],"pubmed_title":["Non-invasive prenatal paternity testing by analysis of Y-chromosome mini-STR haplotype using next-generation sequencing."],"pmcid":["PMC8974964"],"funding_grant_id":["2017RQ169"],"pubmed_authors":["Xia Y","Wang N","Xiao N","Liu M","Zhou S","Shao L","Chen M","Yu W","Zhang L","Pan L","Song W","Duan Y"],"additional_accession":[]},"is_claimable":false,"name":"Non-invasive prenatal paternity testing by analysis of Y-chromosome mini-STR haplotype using next-generation sequencing.","description":"<h4>Objectives</h4>To assess the efficacy of Y-chromosome mini-STR-based next-generation sequencing (NGS) for non-invasive prenatal paternity testing (NIPPT).<h4>Methods</h4>DNA was extracted from the plasma of 24 pregnant women, and cell-free fetal DNA (cffDNA) haplotyping was performed at 12 Y-chromosome mini-STR loci using the Illumina NextSeq 500 system. The cffDNA haplotype was validated by the paternal haplotype. Subsequentlly, the paternity testing parameters were attributed to each case quantitatively.<h4>Results</h4>The biological relationship between the alleged fathers and infants in all 24 family cases were confirmed by capillary electrophoresis (CE). The Y-chromosome mini-STR haplotypes of all 14 male cffDNA were obtained by NGS without any missing loci. The alleles of cffDNA ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-30T15:02:43.222Z","creation":"2025-04-04T23:04:26.861Z"},"accession":"S-EPMC8974964","cross_references":{"pubmed":["35363835"],"doi":["10.1371/journal.pone.0266332"]}}