biostudies-literatureSong WDalian Municipal Science and Technology Bureaue0266332https://www.ebi.ac.uk/biostudies/studies/S-EPMC8974964biostudies-literatureUnknown17(4)<h4>Objectives</h4>To assess the efficacy of Y-chromosome mini-STR-based next-generation sequencing (NGS) for non-invasive prenatal paternity testing (NIPPT).<h4>Methods</h4>DNA was extracted from the plasma of 24 pregnant women, and cell-free fetal DNA (cffDNA) haplotyping was performed at 12 Y-chromosome mini-STR loci using the Illumina NextSeq 500 system. The cffDNA haplotype was validated by the paternal haplotype. Subsequentlly, the paternity testing parameters were attributed to each case quantitatively.<h4>Results</h4>The biological relationship between the alleged fathers and infants in all 24 family cases were confirmed by capillary electrophoresis (CE). The Y-chromosome mini-STR haplotypes of all 14 male cffDNA were obtained by NGS without any missing loci. The alleles of cffDNA PloS oneNon-invasive prenatal paternity testing by analysis of Y-chromosome mini-STR haplotype using next-generation sequencing.PMC89749642017RQ169Xia YWang NXiao NLiu MZhou SShao LChen MYu WZhang LPan LSong WDuan YfalseNon-invasive prenatal paternity testing by analysis of Y-chromosome mini-STR haplotype using next-generation sequencing.<h4>Objectives</h4>To assess the efficacy of Y-chromosome mini-STR-based next-generation sequencing (NGS) for non-invasive prenatal paternity testing (NIPPT).<h4>Methods</h4>DNA was extracted from the plasma of 24 pregnant women, and cell-free fetal DNA (cffDNA) haplotyping was performed at 12 Y-chromosome mini-STR loci using the Illumina NextSeq 500 system. The cffDNA haplotype was validated by the paternal haplotype. Subsequentlly, the paternity testing parameters were attributed to each case quantitatively.<h4>Results</h4>The biological relationship between the alleged fathers and infants in all 24 family cases were confirmed by capillary electrophoresis (CE). The Y-chromosome mini-STR haplotypes of all 14 male cffDNA were obtained by NGS without any missing loci. The alleles of cffDNA 2022-01-01T00:00:00Z20222026-05-30T15:02:43.222Z2025-04-04T23:04:26.861ZS-EPMC89749643536383510.1371/journal.pone.0266332