{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mierzejewska J"],"funding":["Narodowe Centrum Nauki"],"pagination":["7508928"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8975686"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2022"],"pubmed_abstract":["The main purpose of our study was to determine the effect of dendritic cell (DC) transduction with lentiviral vectors carrying sequences of <i>il18</i> and/or <i>il12</i> genes on the level of antitumor activity <i>in vitro</i> and <i>in vivo</i>. We examined the ability of DCs to migrate to the tumor-draining lymph nodes and infiltrate tumor tissue and to activate the local and systemic antitumor response. On the 15th day, DCs genetically modified for production of IL-12 and/or IL-18 were administered peritumorally to C57BL/6 female mice with established MC38 tumors. Lymphoid organs and tumor tissue were collected from mice on the 3rd, 5th, and 7th days after a single administration of DCs for further analysis. Administration of DCs transduced for production of IL-12 alone and in combination with IL-18 increased the inflow and activity of CD4<sup>+</sup> and CD8<sup>+</sup> T lymphocytes in the tumor microenvironment and tumor-draining lymph nodes. We also found that even a single administration of such modified DCs could trigger a systemic antitumor response as well as inhibit tumor growth. Application of the developed DC-based vaccines may exert a favorable impact on stimulation of an antitumor immune response, especially if these DC vaccines are administered repeatedly."],"journal":["Journal of immunology research"],"pubmed_title":["The Beneficial Effect of IL-12 and IL-18 Transduced Dendritic Cells Stimulated with Tumor Antigens on Generation of an Antitumor Response in a Mouse Colon Carcinoma Model."],"pmcid":["PMC8975686"],"funding_grant_id":["2015/17/N/NZ4/02834"],"pubmed_authors":["Mierzejewska J","Szermer-Olearnik B","Wegierek-Ciura K","Rossowska J","Geneja M","Szczygiel A","Pajtasz-Piasecka E","Anger-Gora N"],"additional_accession":[]},"is_claimable":false,"name":"The Beneficial Effect of IL-12 and IL-18 Transduced Dendritic Cells Stimulated with Tumor Antigens on Generation of an Antitumor Response in a Mouse Colon Carcinoma Model.","description":"The main purpose of our study was to determine the effect of dendritic cell (DC) transduction with lentiviral vectors carrying sequences of <i>il18</i> and/or <i>il12</i> genes on the level of antitumor activity <i>in vitro</i> and <i>in vivo</i>. We examined the ability of DCs to migrate to the tumor-draining lymph nodes and infiltrate tumor tissue and to activate the local and systemic antitumor response. On the 15th day, DCs genetically modified for production of IL-12 and/or IL-18 were administered peritumorally to C57BL/6 female mice with established MC38 tumors. Lymphoid organs and tumor tissue were collected from mice on the 3rd, 5th, and 7th days after a single administration of DCs for further analysis. Administration of DCs transduced for production of IL-12 alone and in combination with IL-18 increased the inflow and activity of CD4<sup>+</sup> and CD8<sup>+</sup> T lymphocytes in the tumor microenvironment and tumor-draining lymph nodes. We also found that even a single administration of such modified DCs could trigger a systemic antitumor response as well as inhibit tumor growth. Application of the developed DC-based vaccines may exert a favorable impact on stimulation of an antitumor immune response, especially if these DC vaccines are administered repeatedly.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-19T07:49:13.627Z","creation":"2025-04-19T07:49:13.627Z"},"accession":"S-EPMC8975686","cross_references":{"pubmed":["35372586"],"doi":["10.1155/2022/7508928"]}}