biostudies-literatureChimenti IMinistero della SaluteSapienza Università di RomaMinistero dell'Istruzione, dell'Università e della RicercaSapienza Università di Roma (Sapienza University of Rome)Ministero dell'Istruzione, dell'Università e della Ricerca (Ministry of Education, University and Research)Ministero della Salute (Ministry of Health, Italy)149https://www.ebi.ac.uk/biostudies/studies/S-EPMC8975847biostudies-literatureUnknown8(1)Cardiac stromal cells (CSCs) embrace multiple phenotypes and are a contributory factor in tissue homeostasis and repair. They can be exploited as therapeutic mediators against cardiac fibrosis and remodeling, but their survival and cardioprotective properties can be decreased by microenvironmental cues. We evaluated the impact of autophagy modulation by different pharmacological/genetic approaches on the viability and phenotype of murine CSCs, which had been subjected to nutrient deprivation or hyperglycemia, in order to mimic relevant stress conditions and risk factors of cardiovascular diseases. Our results show that autophagy is activated in CSCs by nutrient deprivation, and that autophagy induction by trehalose or autophagy-related protein 7 (ATG7)-overexpression can significantly presCell death discoveryThe impact of autophagy modulation on phenotype and survival of cardiac stromal cells under metabolic stress.PMC8975847AR120172B8B543B3RG11916B85CDBF762017N8K7S2GR-2013-02355401di Nonno FVersaci FDe Falco ESciarretta SPeruzzi MPicchio VGreco ED'Ambrosio LSchiavon SRubattu SChimenti ISchirone LFrati GPagano FValenti VCalogero AForte MfalseThe impact of autophagy modulation on phenotype and survival of cardiac stromal cells under metabolic stress.Cardiac stromal cells (CSCs) embrace multiple phenotypes and are a contributory factor in tissue homeostasis and repair. They can be exploited as therapeutic mediators against cardiac fibrosis and remodeling, but their survival and cardioprotective properties can be decreased by microenvironmental cues. We evaluated the impact of autophagy modulation by different pharmacological/genetic approaches on the viability and phenotype of murine CSCs, which had been subjected to nutrient deprivation or hyperglycemia, in order to mimic relevant stress conditions and risk factors of cardiovascular diseases. Our results show that autophagy is activated in CSCs by nutrient deprivation, and that autophagy induction by trehalose or autophagy-related protein 7 (ATG7)-overexpression can significantly pres2022-01-01T00:00:00Z2022 Apr2025-04-04T19:40:55.328Z2025-04-04T19:40:55.328ZS-EPMC89758473536562410.1038/s41420-022-00924-7