{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hazleton KZ"],"funding":["NIDDK NIH HHS","NIAID NIH HHS","NCI NIH HHS","NIH HHS"],"pagination":["15"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8975876"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["Clostridioides difficile infection (CDI) is the leading cause of hospital-acquired diarrhea, and emerging evidence has linked dietary components with CDI pathogenesis, suggesting that dietary modulation may be an effective strategy for prevention. Here, we show that mice fed a high-fat/low-fiber \"Western-type\" diet (WD) had dramatically increased mortality in a murine model of antibiotic-induced CDI compared to a low-fat/low-fiber (LF/LF) diet and standard mouse chow controls. We found that the WD had a pro- C. difficile bile acid composition that was driven in part by higher levels of primary bile acids that are produced to digest fat, and a lower level of secondary bile acids that are produced by the gut microbiome. This lack of secondary bile acids was associated with a greater disturbance to the gut microbiome with antibiotics in both the WD and LF/LF diet compared to mouse chow. Mice fed the WD also had the highest level of toxin TcdA just prior to the onset of mortality, but not of TcdB or increased inflammation. These findings indicate that dietary intervention to decrease fat may complement previously proposed dietary intervention strategies to prevent CDI in high-risk individuals."],"journal":["NPJ biofilms and microbiomes"],"pubmed_title":["Dietary fat promotes antibiotic-induced Clostridioides difficile mortality in mice."],"pmcid":["PMC8975876"],"funding_grant_id":["U01 AI150589","P30 DK048520","P30 CA046934","T32 DK067009","T32 AI052066","S10 OD012300","T32 AI007405"],"pubmed_authors":["Martin CG","Reisdorph N","Moreno-Huizar N","Lozupone CA","Armstrong M","Arnolds KL","Hazleton KZ","Orlicky DJ","Nusbacher NM"],"additional_accession":[]},"is_claimable":false,"name":"Dietary fat promotes antibiotic-induced Clostridioides difficile mortality in mice.","description":"Clostridioides difficile infection (CDI) is the leading cause of hospital-acquired diarrhea, and emerging evidence has linked dietary components with CDI pathogenesis, suggesting that dietary modulation may be an effective strategy for prevention. Here, we show that mice fed a high-fat/low-fiber \"Western-type\" diet (WD) had dramatically increased mortality in a murine model of antibiotic-induced CDI compared to a low-fat/low-fiber (LF/LF) diet and standard mouse chow controls. We found that the WD had a pro- C. difficile bile acid composition that was driven in part by higher levels of primary bile acids that are produced to digest fat, and a lower level of secondary bile acids that are produced by the gut microbiome. This lack of secondary bile acids was associated with a greater disturbance to the gut microbiome with antibiotics in both the WD and LF/LF diet compared to mouse chow. Mice fed the WD also had the highest level of toxin TcdA just prior to the onset of mortality, but not of TcdB or increased inflammation. These findings indicate that dietary intervention to decrease fat may complement previously proposed dietary intervention strategies to prevent CDI in high-risk individuals.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-25T18:01:32.337Z","creation":"2025-04-25T18:01:32.337Z"},"accession":"S-EPMC8975876","cross_references":{"pubmed":["35365681"],"doi":["10.1038/s41522-022-00276-1"]}}