{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang SR"],"funding":["BLRD VA","HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases","NIDDK NIH HHS","U.S. Department of Veterans Affairs"],"pagination":["C712-C722"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8977142"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["322(4)"],"pubmed_abstract":["Early gut epithelial restitution reseals superficial wounds after acute injury, but the exact mechanism underlying this rapid mucosal repair remains largely unknown. MicroRNA-195 (miR-195) is highly expressed in the gut epithelium and involved in many aspects of mucosal pathobiology. Actin-related proteins (ARPs) are key components essential for stimulation of actin polymerization and regulate cell motility. Here, we reported that miR-195 modulates early intestinal epithelial restitution by altering ARP-2 expression at the translation level. miR-195 directly interacted with the <i>ARP-2</i> mRNA, and ectopically expressed miR-195 decreased ARP-2 protein without effect on its mRNA content. In contrast, miR-195 silencing by transfection with anti-miR-195 oligo increased ARP-2 expression. Decreased ARP-2 levels by miR-195 overexpression were associated with an inhibition of early epithelial restitution, as indicated by a decrease in cell migration over the wounded area. Elevation of cellular ARP-2 levels by transfection with its transgene restored cell migration after wounding in cells overexpressing miR-195. Polyamines were found to decrease miR-195 abundance and enhanced ARP-2 translation, thus promoting epithelial restitution after wounding. Moreover, increasing the levels of miR-195 disrupted F-actin cytoskeleton organization, which was prevented by ARP2 overexpression. These results indicate that miR-195 inhibits early epithelial restitution by decreasing ARP-2 translation and that miR-195 expression is negatively regulated by cellular polyamines."],"journal":["American journal of physiology. Cell physiology"],"pubmed_title":["miR-195 regulates intestinal epithelial restitution after wounding by altering actin-related protein-2 translation."],"pmcid":["PMC8977142"],"funding_grant_id":["DK57819","DK61972","DK68491","I01 BX000332","R01 DK057819","BX-000713","IK6 BX004213","R01 DK068491","R01 DK061972","BX000332","I01 BX000713"],"pubmed_authors":["Turner DJ","Chung HK","Rao JN","Xiao L","Rathor N","Kwon MS","Wang SR","Wang JY"],"additional_accession":[]},"is_claimable":false,"name":"miR-195 regulates intestinal epithelial restitution after wounding by altering actin-related protein-2 translation.","description":"Early gut epithelial restitution reseals superficial wounds after acute injury, but the exact mechanism underlying this rapid mucosal repair remains largely unknown. MicroRNA-195 (miR-195) is highly expressed in the gut epithelium and involved in many aspects of mucosal pathobiology. Actin-related proteins (ARPs) are key components essential for stimulation of actin polymerization and regulate cell motility. Here, we reported that miR-195 modulates early intestinal epithelial restitution by altering ARP-2 expression at the translation level. miR-195 directly interacted with the <i>ARP-2</i> mRNA, and ectopically expressed miR-195 decreased ARP-2 protein without effect on its mRNA content. In contrast, miR-195 silencing by transfection with anti-miR-195 oligo increased ARP-2 expression. Decreased ARP-2 levels by miR-195 overexpression were associated with an inhibition of early epithelial restitution, as indicated by a decrease in cell migration over the wounded area. Elevation of cellular ARP-2 levels by transfection with its transgene restored cell migration after wounding in cells overexpressing miR-195. Polyamines were found to decrease miR-195 abundance and enhanced ARP-2 translation, thus promoting epithelial restitution after wounding. Moreover, increasing the levels of miR-195 disrupted F-actin cytoskeleton organization, which was prevented by ARP2 overexpression. These results indicate that miR-195 inhibits early epithelial restitution by decreasing ARP-2 translation and that miR-195 expression is negatively regulated by cellular polyamines.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-26T04:51:38.705Z","creation":"2025-04-06T11:13:49.414Z"},"accession":"S-EPMC8977142","cross_references":{"pubmed":["35235424"],"doi":["10.1152/ajpcell.00001.2022"]}}