{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xiong X"],"funding":["NICHD NIH HHS","NIBIB NIH HHS","NCRR NIH HHS","NIDDK NIH HHS","NIAID NIH HHS","NHLBI NIH HHS","NINDS NIH HHS","NIGMS NIH HHS"],"pagination":["1157-1171.e22"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8978092"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["185(7)"],"pubmed_abstract":["Enterococci are a part of human microbiota and a leading cause of multidrug resistant infections. Here, we identify a family of Enterococcus pore-forming toxins (Epxs) in E. faecalis, E. faecium, and E. hirae strains isolated across the globe. Structural studies reveal that Epxs form a branch of β-barrel pore-forming toxins with a β-barrel protrusion (designated the top domain) sitting atop the cap domain. Through a genome-wide CRISPR-Cas9 screen, we identify human leukocyte antigen class I (HLA-I) complex as a receptor for two members (Epx2 and Epx3), which preferentially recognize human HLA-I and homologous MHC-I of equine, bovine, and porcine, but not murine, origin. Interferon exposure, which stimulates MHC-I expression, sensitizes human cells and intestinal organoids to Epx2 and Epx3 "],"journal":["Cell"],"pubmed_title":["Emerging enterococcus pore-forming toxins with MHC/HLA-I as receptors."],"pmcid":["PMC8978092"],"funding_grant_id":["R01 HL093242","R01 AI158503","P30 GM124165","R01 HL130845","P01 AI083214","R01 AI132387","R01 HL137229","R01 EB021908","T32 HL007917","R01 AI139087","P30 HD018655","R01 NS080833","R21 NS106159","HHSN272200900018C","P30 DK034854","R01 HL156362","R01 NS117626","S10 RR029205","DP2 GM140920","U19 AI110818"],"pubmed_authors":["Chen P","Zhang J","Dong M","Qi W","Abraham J","Lebreton F","Chen H","Xiong X","Wu H","Ruan J","Gilmore MS","Sheng K","Breault DT","Bao H","Yang P","Tian S","Earl AM","Yin L"],"additional_accession":[]},"is_claimable":false,"name":"Emerging enterococcus pore-forming toxins with MHC/HLA-I as receptors.","description":"Enterococci are a part of human microbiota and a leading cause of multidrug resistant infections. Here, we identify a family of Enterococcus pore-forming toxins (Epxs) in E. faecalis, E. faecium, and E. hirae strains isolated across the globe. Structural studies reveal that Epxs form a branch of β-barrel pore-forming toxins with a β-barrel protrusion (designated the top domain) sitting atop the cap domain. Through a genome-wide CRISPR-Cas9 screen, we identify human leukocyte antigen class I (HLA-I) complex as a receptor for two members (Epx2 and Epx3), which preferentially recognize human HLA-I and homologous MHC-I of equine, bovine, and porcine, but not murine, origin. Interferon exposure, which stimulates MHC-I expression, sensitizes human cells and intestinal organoids to Epx2 and Epx3 ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-19T13:32:28.552Z","creation":"2024-11-15T19:24:42.351Z"},"accession":"S-EPMC8978092","cross_references":{"pubmed":["35259335"],"doi":["10.1016/j.cell.2022.02.002"]}}