biostudies-literatureUnknown86(2)Dong JVesicular disease caused by Seneca Valley virus (SVV) has recently emerged throughout China and caused certain industry losses. We used immunofluorescence and western blotting to confirm that 3 new SVV strains (CH-GDSG-2018-1, CH-GDSG-2018-2, and CH-GDSG-2018-3) were from 1 pig farm. Phylogenetic analysis revealed the following: i) all 3 strains belong to USA-GBI29-2015-like clades, ii) CH-GDSG-2018-3 might have diverged from CH-GDSG-2018-1 and CH-GDSG-2018-2, and iii) CH-GDSG-2018-3 is a recombinant of the CHhb17 and HeNKF-1 strains. Virus growth curves showed that CH-GDSG-2018-3 had stronger proliferation ability <i>in vitro</i>. Seneca Valley virus has evolved extensively within China and this study has furthered our understanding of SVV epidemiology.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire102-107https://www.ebi.ac.uk/biostudies/studies/S-EPMC8978293biostudies-literatureEvolutionary analysis of 3 isolates of Seneca Valley virus from a single pig farm.PMC8978293He SRao DGeng XChen MDong JZhao PJiao FHuang LChen BCong FfalseEvolutionary analysis of 3 isolates of Seneca Valley virus from a single pig farm.Vesicular disease caused by Seneca Valley virus (SVV) has recently emerged throughout China and caused certain industry losses. We used immunofluorescence and western blotting to confirm that 3 new SVV strains (CH-GDSG-2018-1, CH-GDSG-2018-2, and CH-GDSG-2018-3) were from 1 pig farm. Phylogenetic analysis revealed the following: i) all 3 strains belong to USA-GBI29-2015-like clades, ii) CH-GDSG-2018-3 might have diverged from CH-GDSG-2018-1 and CH-GDSG-2018-2, and iii) CH-GDSG-2018-3 is a recombinant of the CHhb17 and HeNKF-1 strains. Virus growth curves showed that CH-GDSG-2018-3 had stronger proliferation ability <i>in vitro</i>. Seneca Valley virus has evolved extensively within China and this study has furthered our understanding of SVV epidemiology.2022-01-01T00:00:00Z2022 Apr2025-04-22T21:52:01.433Z2025-02-19T01:27:44.613ZS-EPMC897829335388228