biostudies-literatureWen JBasic and Applied Basic Research Foundation of Guangdong Province1675-1681https://www.ebi.ac.uk/biostudies/studies/S-EPMC8978865biostudies-literatureUnknown12(3)Rapid measurement of waterborne bacterial viability is crucial for ensuring the safety of public health. Herein, we proposed a colorimetric assay for rapid measurement of waterborne bacterial viability based on a difunctional gold nanoprobe (dGNP). This versatile dGNP is composed of bacteria recognizing parts and signal indicating parts, and can generate color signals while recognizing bacterial suspensions of different viabilities. This dGNP-based colorimetric assay has a fast response and can be accomplished within 10 min. Moreover, the proposed colorimetric method is able to measure bacterial viability between 0% and 100%. The method can also measure the viability of other bacteria including <i>Staphylococcus aureus</i>, <i>Shewanella oneidensis</i>, and <i>Escherichia coli</i> O157H7. RSC advancesRapid measurement of waterborne bacterial viability based on difunctional gold nanoprobe.PMC89788652021A1515010173Wu JLiu JWen JHe DfalseRapid measurement of waterborne bacterial viability based on difunctional gold nanoprobe.Rapid measurement of waterborne bacterial viability is crucial for ensuring the safety of public health. Herein, we proposed a colorimetric assay for rapid measurement of waterborne bacterial viability based on a difunctional gold nanoprobe (dGNP). This versatile dGNP is composed of bacteria recognizing parts and signal indicating parts, and can generate color signals while recognizing bacterial suspensions of different viabilities. This dGNP-based colorimetric assay has a fast response and can be accomplished within 10 min. Moreover, the proposed colorimetric method is able to measure bacterial viability between 0% and 100%. The method can also measure the viability of other bacteria including <i>Staphylococcus aureus</i>, <i>Shewanella oneidensis</i>, and <i>Escherichia coli</i> O157H7. 2022-01-01T00:00:00Z2022 Jan2025-04-19T13:01:43.504Z2025-04-19T13:01:43.504ZS-EPMC89788653542516110.1039/d1ra07287k