biostudies-literatureUnknown9Tan Y<h4>Introduction</h4>Metabolic acidosis affects bone health. It remains unclear whether drinking natural mineral water is better for maintaining bone health in the youth with metabolic acidosis.<h4>Materials and methods</h4>Sixty young female rats (3-weeks-old) were randomly divided into three groups and drank purified water (PW, as control), bicarbonate-rich natural mineral water (Bic-NMW), or sulfate-rich natural mineral water (Sul-NMW), which, respectively, contained calcium (0.17, 155, and 175 mg/L), bicarbonate (0.1360, and 139 mg/L) and sulfate (0, 35.6, and 532 mg/L), for 16 weeks. In the last 3 weeks, metabolic acidosis was induced in 10 rats per group by adding NH₄Cl (0.28 mM) to drinking water. The rats' blood, urine, and femur were collected for assessing acid-base stFrontiers in nutrition813202https://www.ebi.ac.uk/biostudies/studies/S-EPMC8979287biostudies-literatureDrinking Natural Mineral Water Maintains Bone Health in Young Rats With Metabolic Acidosis.PMC8979287Qiu ZJin HHuang YLuo JShu WTan YXu AZeng HWang YXue JWang JWang LfalseDrinking Natural Mineral Water Maintains Bone Health in Young Rats With Metabolic Acidosis.<h4>Introduction</h4>Metabolic acidosis affects bone health. It remains unclear whether drinking natural mineral water is better for maintaining bone health in the youth with metabolic acidosis.<h4>Materials and methods</h4>Sixty young female rats (3-weeks-old) were randomly divided into three groups and drank purified water (PW, as control), bicarbonate-rich natural mineral water (Bic-NMW), or sulfate-rich natural mineral water (Sul-NMW), which, respectively, contained calcium (0.17, 155, and 175 mg/L), bicarbonate (0.1360, and 139 mg/L) and sulfate (0, 35.6, and 532 mg/L), for 16 weeks. In the last 3 weeks, metabolic acidosis was induced in 10 rats per group by adding NH₄Cl (0.28 mM) to drinking water. The rats' blood, urine, and femur were collected for assessing acid-base st2022-01-01T00:00:00Z20222025-04-05T15:52:45.733Z2025-04-05T15:52:45.733ZS-EPMC89792873538719610.3389/fnut.2022.813202