{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10"],"submitter":["Chen H"],"funding":["Sick Kids Foundation"],"pubmed_abstract":["Following their production in the testis, spermatozoa enter the epididymis where they gain their motility and fertilizing abilities. This post-testicular maturation coincides with sperm epigenetic profile changes that influence progeny outcome. While recent studies highlighted the dynamics of small non-coding RNAs in maturing spermatozoa, little is known regarding sperm methylation changes and their impact at the post-fertilization level. Fluorescence-activated cell sorting (FACS) was used to purify spermatozoa from the testis and different epididymal segments (i.e., <i>caput, corpus and cauda</i>) of CAG/su9-DsRed2; Acr3-EGFP transgenic mice in order to map out sperm methylome dynamics. Reduced representation bisulfite sequencing (RRBS-Seq) performed on DNA from these respective sperm pop"],"journal":["Frontiers in cell and developmental biology"],"pagination":["834519"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8981467"],"repository":["biostudies-literature"],"pubmed_title":["Sperm Heterogeneity Accounts for Sperm DNA Methylation Variations Observed in the Caput Epididymis, Independently From DNMT/TET Activities."],"pmcid":["PMC8981467"],"pubmed_authors":["Droit A","Scott-Boyer MP","Belleannee C","Robert C","Chen H"],"additional_accession":[]},"is_claimable":false,"name":"Sperm Heterogeneity Accounts for Sperm DNA Methylation Variations Observed in the Caput Epididymis, Independently From DNMT/TET Activities.","description":"Following their production in the testis, spermatozoa enter the epididymis where they gain their motility and fertilizing abilities. This post-testicular maturation coincides with sperm epigenetic profile changes that influence progeny outcome. While recent studies highlighted the dynamics of small non-coding RNAs in maturing spermatozoa, little is known regarding sperm methylation changes and their impact at the post-fertilization level. Fluorescence-activated cell sorting (FACS) was used to purify spermatozoa from the testis and different epididymal segments (i.e., <i>caput, corpus and cauda</i>) of CAG/su9-DsRed2; Acr3-EGFP transgenic mice in order to map out sperm methylome dynamics. Reduced representation bisulfite sequencing (RRBS-Seq) performed on DNA from these respective sperm pop","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-31T13:00:06.477Z","creation":"2025-04-06T21:51:47.223Z"},"accession":"S-EPMC8981467","cross_references":{"pubmed":["35392175"],"doi":["10.3389/fcell.2022.834519"]}}