{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kirkemo LL"],"funding":["Chan Zuckerberg Biohub Investigator Program","National Cancer Institute","NCI NIH HHS","National Institute of General Medical Sciences","Harry and Dianna Hind Professorship","NIGMS NIH HHS","National Science Foundation"],"pagination":["e73982"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8983049"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11"],"pubmed_abstract":["Characterization of cell surface proteome differences between cancer and healthy cells is a valuable approach for the identification of novel diagnostic and therapeutic targets. However, selective sampling of surface proteins for proteomics requires large samples (>10e6 cells) and long labeling times. These limitations preclude analysis of material-limited biological samples or the capture of rapid surface proteomic changes. Here, we present two labeling approaches to tether exogenous peroxidases (APEX2 and HRP) directly to cells, enabling rapid, small-scale cell surface biotinylation without the need to engineer cells. We used a novel lipidated DNA-tethered APEX2 (DNA-APEX2), which upon addition to cells promoted cell agnostic membrane-proximal labeling. Alternatively, we employed horsera"],"journal":["eLife"],"pubmed_title":["Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cells."],"pmcid":["PMC8983049"],"funding_grant_id":["R35 GM122451","U01 CA244452","F31 CA247527","R01CA248323","1F31CA247527","U01CA244452","R01 CA248323","1650113","R35GM122451"],"pubmed_authors":["Blelloch R","Kirkemo LL","Yang J","Glasgow JE","Byrnes JR","Elledge SK","Wells JA"],"additional_accession":[]},"is_claimable":false,"name":"Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cells.","description":"Characterization of cell surface proteome differences between cancer and healthy cells is a valuable approach for the identification of novel diagnostic and therapeutic targets. However, selective sampling of surface proteins for proteomics requires large samples (>10e6 cells) and long labeling times. These limitations preclude analysis of material-limited biological samples or the capture of rapid surface proteomic changes. Here, we present two labeling approaches to tether exogenous peroxidases (APEX2 and HRP) directly to cells, enabling rapid, small-scale cell surface biotinylation without the need to engineer cells. We used a novel lipidated DNA-tethered APEX2 (DNA-APEX2), which upon addition to cells promoted cell agnostic membrane-proximal labeling. Alternatively, we employed horsera","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-05-31T01:04:14.913Z","creation":"2025-04-04T11:31:49.19Z"},"accession":"S-EPMC8983049","cross_references":{"pubmed":["35257663"],"doi":["10.7554/eLife.73982"]}}