biostudies-literatureJin XSouthwest Medical University3998338https://www.ebi.ac.uk/biostudies/studies/S-EPMC8983225biostudies-literatureUnknown2022Simple, rapid, and accurate detection of myoinositol (MI) concentration in blood is crucial in diagnosing polycystic ovary syndrome, neurological disorders, and cancer. A novel electrochemical detection (IED) method was established to quantify MI in human serum using a disposable unmodified screen-printed carbon electrode (SPCE) for the first time. MI was detected indirectly by the reaction product of myoinositol dehydrogenase (IDH) and cofactor <i>β</i>-nicotinamide adenine dinucleotide (NAD⁺). Good linear calibration curves were obtained at the concentration range from 5.0 <i>μ</i>M to 500.0 <i>μ</i>M (<i>R</i> ² = 0.9981) with the lower limits of detection (LOD) and quantification (LOQ) of 1.0 <i>μ</i>M and 2.5 <i>μ</i>M, respectively. Recoveries were calculated atJournal of analytical methods in chemistryQuantification of Myoinositol in Serum by Electrochemical Detection with an Unmodified Screen-Printed Carbon Electrode.PMC89832252019YFS03322019YFH001019PJ2932021ZKQN063Li GLiu JJin XGu XTian GZhao YKong XZhong MfalseQuantification of Myoinositol in Serum by Electrochemical Detection with an Unmodified Screen-Printed Carbon Electrode.Simple, rapid, and accurate detection of myoinositol (MI) concentration in blood is crucial in diagnosing polycystic ovary syndrome, neurological disorders, and cancer. A novel electrochemical detection (IED) method was established to quantify MI in human serum using a disposable unmodified screen-printed carbon electrode (SPCE) for the first time. MI was detected indirectly by the reaction product of myoinositol dehydrogenase (IDH) and cofactor <i>β</i>-nicotinamide adenine dinucleotide (NAD⁺). Good linear calibration curves were obtained at the concentration range from 5.0 <i>μ</i>M to 500.0 <i>μ</i>M (<i>R</i> ² = 0.9981) with the lower limits of detection (LOD) and quantification (LOQ) of 1.0 <i>μ</i>M and 2.5 <i>μ</i>M, respectively. Recoveries were calculated at2022-01-01T00:00:00Z20222025-04-04T12:57:42.083Z2025-04-04T12:57:42.083ZS-EPMC89832253539228110.1155/2022/3998338