biostudies-literatureUnknown13Hu YNational Natural Science Foundation of China<b>Background:</b> Pilose antler peptide (PAP), prepared from the pilose antler of <i>Cervus nippon</i> Temminck, is widely used in traditional Chinese medicine (TCM) against various inflammatory disorders. TCM prescriptions containing pilose antler are often prescribed clinically to treat depression. However, the pharmacological mechanisms of how PAP, against inflammation, prevents and treats depression remain poorly understood. <b>Methods:</b> PAP was identified by <i>de novo</i> sequencing and database searching. Then, behavioral tests were conducted to investigate the effect of PAP on CUMS-exposed mice. In parallel, Nissl staining and Golgi-Cox staining were used for exploring the effect of PAP on neural cells and dendritic spine density. Additionally, the expression of key proteins ofFrontiers in pharmacology815413https://www.ebi.ac.uk/biostudies/studies/S-EPMC8984150biostudies-literatureThe Protective Effect of Pilose Antler Peptide on CUMS-Induced Depression Through AMPK/Sirt1/NF-κB/NLRP3-Mediated Pyroptosis.PMC8984150Zhao MQi MHu YDong YZhao TYao GfalseThe Protective Effect of Pilose Antler Peptide on CUMS-Induced Depression Through AMPK/Sirt1/NF-κB/NLRP3-Mediated Pyroptosis.<b>Background:</b> Pilose antler peptide (PAP), prepared from the pilose antler of <i>Cervus nippon</i> Temminck, is widely used in traditional Chinese medicine (TCM) against various inflammatory disorders. TCM prescriptions containing pilose antler are often prescribed clinically to treat depression. However, the pharmacological mechanisms of how PAP, against inflammation, prevents and treats depression remain poorly understood. <b>Methods:</b> PAP was identified by <i>de novo</i> sequencing and database searching. Then, behavioral tests were conducted to investigate the effect of PAP on CUMS-exposed mice. In parallel, Nissl staining and Golgi-Cox staining were used for exploring the effect of PAP on neural cells and dendritic spine density. Additionally, the expression of key proteins of2022-01-01T00:00:00Z20222025-04-27T01:09:28.827Z2025-04-06T18:07:32.947ZS-EPMC89841503540122610.3389/fphar.2022.815413