biostudies-literatureUnknown3(4)Feng HWe study and uncover the effect of hairpin structures in loops of G-quadruplexes using spectroscopic methods. Notably, we show that the sequence, structure, and position of the hairpin loop control the spectroscopic properties of long loop G-quadruplexes, and highlight that intrinsic fluorescence can be used to monitor the formation of non-canonical G-quadruplexes.RSC chemical biology431-435https://www.ebi.ac.uk/biostudies/studies/S-EPMC8984947biostudies-literatureSpectroscopic analysis reveals the effect of hairpin loop formation on G-quadruplex structures.PMC8984947Kwok CKFeng HfalseSpectroscopic analysis reveals the effect of hairpin loop formation on G-quadruplex structures.We study and uncover the effect of hairpin structures in loops of G-quadruplexes using spectroscopic methods. Notably, we show that the sequence, structure, and position of the hairpin loop control the spectroscopic properties of long loop G-quadruplexes, and highlight that intrinsic fluorescence can be used to monitor the formation of non-canonical G-quadruplexes.2022-01-01T00:00:00Z2022 Apr2025-04-04T20:37:20.306Z2025-04-04T20:37:20.306ZS-EPMC89849473544114010.1039/d2cb00045h