{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(3)"],"submitter":["Ma Q"],"pubmed_abstract":["<h4>Background</h4>In tumors, the role of human antigen R (<i>HuR</i>) includes regulating tumor cell proliferation, differentiation, apoptosis, angiogenesis, and lymphangiogenesis. Previous studies have revealed that the expression of <i>HuR</i> can be detected in bladder cancer, and is related to the biological behavior of malignancy.<h4>Methods</h4>T24 cells were transfected by <i>HuR</i> overexpression and <i>HuR</i> knockdown vectors, and divided into the control group, the overexpression-<i>HuR</i> group, and the cas9-<i>HuR</i> group. Cell viability was detected after 48 h by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay, apoptosis was detected by Annexin V-allophycocyanin (APC)/7-aminoactinomycin D (7-AAD) double staining, cell migration was detected by Transwell assays, and the expression levels of <i>HuR</i>, <i>cyclin D1</i>, and apoptosis-related factors [i.e., <i>B-cell lymphoma 2</i> (<i>Bcl-2</i>)] were detected by fluorescence quantitative polymerase chain reaction (PCR) and Western blot.<h4>Results</h4>Compared to the control group, cell viability after 48 h increased significantly in the overexpression-<i>HuR</i> group, and decreased significantly in the cas9-<i>HuR</i> group (P<0.05). The number of migrating cells increased significantly in the overexpression-<i>HuR</i> group, and decreased significantly in the cas9-<i>HuR</i> group (P<0.05). The apoptosis rate was significantly decreased in the overexpression-<i>HuR</i> group, and significantly increased in the cas9-<i>HuR</i> group (P<0.05). The messenger ribonucleic acid and protein expression levels of <i>HuR</i>, <i>cyclin D1</i>, and <i>Bcl-2</i> were significantly increased in the overexpression-<i>HuR</i> group, and significantly decreased in the cas9-<i>HuR</i> group (P<0.05).<h4>Conclusions</h4><i>HuR</i> promotes the proliferation and migration of T24 cells, and inhibits cell apoptosis. The mechanism may be related to the expression of <i>cyclin D</i> and the apoptosis-related protein, Bcl-2."],"journal":["Translational andrology and urology"],"pagination":["348-357"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8984971"],"repository":["biostudies-literature"],"pubmed_title":["The effects of modified RNA-binding proteins HuR on the biological behavior of the bladder cancer T24 cell line."],"pmcid":["PMC8984971"],"pubmed_authors":["Ma Q","Xu C","Han X","Wu F","Zhang W","Liu X","Zheng K","Liu Z","Zhang T","Wu R","Wang X","Su Y","Wang Y"],"additional_accession":[]},"is_claimable":false,"name":"The effects of modified RNA-binding proteins HuR on the biological behavior of the bladder cancer T24 cell line.","description":"<h4>Background</h4>In tumors, the role of human antigen R (<i>HuR</i>) includes regulating tumor cell proliferation, differentiation, apoptosis, angiogenesis, and lymphangiogenesis. Previous studies have revealed that the expression of <i>HuR</i> can be detected in bladder cancer, and is related to the biological behavior of malignancy.<h4>Methods</h4>T24 cells were transfected by <i>HuR</i> overexpression and <i>HuR</i> knockdown vectors, and divided into the control group, the overexpression-<i>HuR</i> group, and the cas9-<i>HuR</i> group. Cell viability was detected after 48 h by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay, apoptosis was detected by Annexin V-allophycocyanin (APC)/7-aminoactinomycin D (7-AAD) double staining, cell migration was detected by Transwell assays, and the expression levels of <i>HuR</i>, <i>cyclin D1</i>, and apoptosis-related factors [i.e., <i>B-cell lymphoma 2</i> (<i>Bcl-2</i>)] were detected by fluorescence quantitative polymerase chain reaction (PCR) and Western blot.<h4>Results</h4>Compared to the control group, cell viability after 48 h increased significantly in the overexpression-<i>HuR</i> group, and decreased significantly in the cas9-<i>HuR</i> group (P<0.05). The number of migrating cells increased significantly in the overexpression-<i>HuR</i> group, and decreased significantly in the cas9-<i>HuR</i> group (P<0.05). The apoptosis rate was significantly decreased in the overexpression-<i>HuR</i> group, and significantly increased in the cas9-<i>HuR</i> group (P<0.05). The messenger ribonucleic acid and protein expression levels of <i>HuR</i>, <i>cyclin D1</i>, and <i>Bcl-2</i> were significantly increased in the overexpression-<i>HuR</i> group, and significantly decreased in the cas9-<i>HuR</i> group (P<0.05).<h4>Conclusions</h4><i>HuR</i> promotes the proliferation and migration of T24 cells, and inhibits cell apoptosis. The mechanism may be related to the expression of <i>cyclin D</i> and the apoptosis-related protein, Bcl-2.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-05T16:07:16.373Z","creation":"2025-04-05T16:07:16.373Z"},"accession":"S-EPMC8984971","cross_references":{"pubmed":["35402198"],"doi":["10.21037/tau-22-123"]}}