biostudies-literatureFerro LSHoward Hughes Medical InstituteNIGMS NIH HHS326-331https://www.ebi.ac.uk/biostudies/studies/S-EPMC8985661biostudies-literatureUnknown375(6578)Microtubule (MT)-associated protein 7 (MAP7) is a required cofactor for kinesin-1-driven transport of intracellular cargoes. Using cryo-electron microscopy and single-molecule imaging, we investigated how MAP7 binds MTs and facilitates kinesin-1 motility. The MT-binding domain (MTBD) of MAP7 bound MTs as an extended α helix between the protofilament ridge and the site of lateral contact. Unexpectedly, the MTBD partially overlapped with the binding site of kinesin-1 and inhibited its motility. However, by tethering kinesin-1 to the MT, the projection domain of MAP7 prevented dissociation of the motor and facilitated its binding to available neighboring sites. The inhibitory effect of the MTBD dominated as MTs became saturated with MAP7. Our results reveal biphasic regulation of kinesin-1 byScience (New York, N.Y.)Structural and functional insight into regulation of kinesin-1 by microtubule-associated protein MAP7.PMC8985661R35 GM127018R01 GM094522T32 GM008295P01 GM051487R35 GM136414R01 GM123089F31 GM123655Nogales ECosta KFernandes JEshun-Wilson LJack AFarrell DPGur MDiMaio FYildiz AFang QHuijben TFerro LSGolcuk MfalseStructural and functional insight into regulation of kinesin-1 by microtubule-associated protein MAP7.Microtubule (MT)-associated protein 7 (MAP7) is a required cofactor for kinesin-1-driven transport of intracellular cargoes. Using cryo-electron microscopy and single-molecule imaging, we investigated how MAP7 binds MTs and facilitates kinesin-1 motility. The MT-binding domain (MTBD) of MAP7 bound MTs as an extended α helix between the protofilament ridge and the site of lateral contact. Unexpectedly, the MTBD partially overlapped with the binding site of kinesin-1 and inhibited its motility. However, by tethering kinesin-1 to the MT, the projection domain of MAP7 prevented dissociation of the motor and facilitated its binding to available neighboring sites. The inhibitory effect of the MTBD dominated as MTs became saturated with MAP7. Our results reveal biphasic regulation of kinesin-1 by2022-01-01T00:00:00Z2022 Jan2026-05-09T23:45:12.233Z2025-04-04T22:14:56.947ZS-EPMC89856613505065710.1126/science.abf6154