{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ziegler P"],"funding":["NCI NIH HHS"],"pagination":["e4365"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8986362"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(6)"],"pubmed_abstract":["The ubiquitous and cancer-associated Epstein-Barr virus (EBV) is associated with nearly all cases of nasopharyngeal carcinoma (NPC). Nasopharyngeal tissue is comprised of both pseudostratified and stratified epithelium, which are modeled in three-dimensional (3-D) cell culture. The cellular origin of EBV-associated NPC is as yet unknown, but both latent and lytic infections are likely important for preneoplastic mechanisms and replenishing the compartmentalized viral reservoir. Conventional 2-D cultures of nasopharyngeal epithelial cells (as primary cells or immortalized cell lines) are difficult to infect with EBV and cannot mimic the tissue-specific biology of the airway epithelium, which can only be captured in 3-D models. We have shown that EBV can infect the pseudostratified epitheliu"],"journal":["Bio-protocol"],"pubmed_title":["Three-dimensional Models of the Nasopharynx for the Study of Epstein-Barr Virus Infection."],"pmcid":["PMC8986362"],"funding_grant_id":["P30 CA047904"],"pubmed_authors":["Sample CE","Reznik AS","Kitchloo SP","Green A","Wang E","Lee SE","Shair KHY","Myerburg MM","Ziegler P"],"additional_accession":[]},"is_claimable":false,"name":"Three-dimensional Models of the Nasopharynx for the Study of Epstein-Barr Virus Infection.","description":"The ubiquitous and cancer-associated Epstein-Barr virus (EBV) is associated with nearly all cases of nasopharyngeal carcinoma (NPC). Nasopharyngeal tissue is comprised of both pseudostratified and stratified epithelium, which are modeled in three-dimensional (3-D) cell culture. The cellular origin of EBV-associated NPC is as yet unknown, but both latent and lytic infections are likely important for preneoplastic mechanisms and replenishing the compartmentalized viral reservoir. Conventional 2-D cultures of nasopharyngeal epithelial cells (as primary cells or immortalized cell lines) are difficult to infect with EBV and cannot mimic the tissue-specific biology of the airway epithelium, which can only be captured in 3-D models. We have shown that EBV can infect the pseudostratified epitheliu","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2026-06-12T03:19:23.102Z","creation":"2024-11-19T17:29:10.931Z"},"accession":"S-EPMC8986362","cross_references":{"pubmed":["35434197"],"doi":["10.21769/BioProtoc.4365","10.21769/bioprotoc.4365"]}}