{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Mudrik-Zohar H"],"funding":["Technion-Israel Institute of Technology","German-Israeli Foundation for Scientific Research and Development","Seerave Foundation"],"pubmed_abstract":["<h4>Background</h4>Infected diabetic foot ulcers (IDFU) are a major complication of diabetes mellitus. These potentially limb-threatening ulcers are challenging to treat due to impaired wound healing characterizing diabetic patients and the complex microbial environment of these ulcers.<h4>Aim</h4>To analyze the microbiome of IDFU in association with clinical outcomes.<h4>Methods</h4>Wound biopsies from IDFU were obtained from hospitalized patients and were analyzed using traditional microbiology cultures, 16S rRNA sequencing and metagenomic sequencing. Patients' characteristics, culture-based results and sequencing data were analyzed in association with clinical outcomes.<h4>Results</h4>A total of 31 patients were enrolled. Gram-negative bacteria dominated the IDFU samples (79%, 59% and 5"],"journal":["Frontiers in cellular and infection microbiology"],"pagination":["836699"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8987016"],"repository":["biostudies-literature"],"pubmed_title":["Microbiome Characterization of Infected Diabetic Foot Ulcers in Association With Clinical Outcomes: Traditional Cultures Versus Molecular Sequencing Methods."],"pmcid":["PMC8987016"],"pubmed_authors":["Mudrik-Zohar H","Carasso S","Gefen T","Paitan Y","Zalmanovich A","Cohen Y","Chowers M","Katzir M","Geva-Zatorsky N"],"additional_accession":[]},"is_claimable":false,"name":"Microbiome Characterization of Infected Diabetic Foot Ulcers in Association With Clinical Outcomes: Traditional Cultures Versus Molecular Sequencing Methods.","description":"<h4>Background</h4>Infected diabetic foot ulcers (IDFU) are a major complication of diabetes mellitus. These potentially limb-threatening ulcers are challenging to treat due to impaired wound healing characterizing diabetic patients and the complex microbial environment of these ulcers.<h4>Aim</h4>To analyze the microbiome of IDFU in association with clinical outcomes.<h4>Methods</h4>Wound biopsies from IDFU were obtained from hospitalized patients and were analyzed using traditional microbiology cultures, 16S rRNA sequencing and metagenomic sequencing. Patients' characteristics, culture-based results and sequencing data were analyzed in association with clinical outcomes.<h4>Results</h4>A total of 31 patients were enrolled. Gram-negative bacteria dominated the IDFU samples (79%, 59% and 5","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-31T20:01:50.299Z","creation":"2024-12-03T22:56:30.973Z"},"accession":"S-EPMC8987016","cross_references":{"pubmed":["35402307"],"doi":["10.3389/fcimb.2022.836699"]}}