biostudies-literatureUnknown12Mudrik-Zohar HTechnion-Israel Institute of TechnologyGerman-Israeli Foundation for Scientific Research and DevelopmentSeerave Foundation<h4>Background</h4>Infected diabetic foot ulcers (IDFU) are a major complication of diabetes mellitus. These potentially limb-threatening ulcers are challenging to treat due to impaired wound healing characterizing diabetic patients and the complex microbial environment of these ulcers.<h4>Aim</h4>To analyze the microbiome of IDFU in association with clinical outcomes.<h4>Methods</h4>Wound biopsies from IDFU were obtained from hospitalized patients and were analyzed using traditional microbiology cultures, 16S rRNA sequencing and metagenomic sequencing. Patients' characteristics, culture-based results and sequencing data were analyzed in association with clinical outcomes.<h4>Results</h4>A total of 31 patients were enrolled. Gram-negative bacteria dominated the IDFU samples (79%, 59% and 5Frontiers in cellular and infection microbiology836699https://www.ebi.ac.uk/biostudies/studies/S-EPMC8987016biostudies-literatureMicrobiome Characterization of Infected Diabetic Foot Ulcers in Association With Clinical Outcomes: Traditional Cultures Versus Molecular Sequencing Methods.PMC8987016Mudrik-Zohar HCarasso SGefen TPaitan YZalmanovich ACohen YChowers MKatzir MGeva-Zatorsky NfalseMicrobiome Characterization of Infected Diabetic Foot Ulcers in Association With Clinical Outcomes: Traditional Cultures Versus Molecular Sequencing Methods.<h4>Background</h4>Infected diabetic foot ulcers (IDFU) are a major complication of diabetes mellitus. These potentially limb-threatening ulcers are challenging to treat due to impaired wound healing characterizing diabetic patients and the complex microbial environment of these ulcers.<h4>Aim</h4>To analyze the microbiome of IDFU in association with clinical outcomes.<h4>Methods</h4>Wound biopsies from IDFU were obtained from hospitalized patients and were analyzed using traditional microbiology cultures, 16S rRNA sequencing and metagenomic sequencing. Patients' characteristics, culture-based results and sequencing data were analyzed in association with clinical outcomes.<h4>Results</h4>A total of 31 patients were enrolled. Gram-negative bacteria dominated the IDFU samples (79%, 59% and 52022-01-01T00:00:00Z20222026-05-31T20:01:50.299Z2024-12-03T22:56:30.973ZS-EPMC89870163540230710.3389/fcimb.2022.836699